DVD technology-based molecular diagnosis platform: quantitative pregnancy test on a disc.
DVD technology-based molecular diagnosis platform: quantitative pregnancy test on a disc.
复制标题
DOI：
10.1039/c3lc51411k
复制
发表时间：
2014-04
期刊：
影响因子：
6.1
通讯作者：
Xiaochun Li;S. Weng;B. Ge;Zhihui Yao;Hua-Zhong Yu
中科院分区：
文献类型：
--
作者：
Xiaochun Li;S. Weng;B. Ge;Zhihui Yao;Hua-Zhong Yu
开发了一个完全基于DVD技术的诊断平台，用于现场定量检测感兴趣的分子分析物，例如尿样中的人绒毛膜促性腺激素(HCG)(“盘上定量妊娠试验”)。在常规DVD-R上制备的hCG特异性单抗结合试验用纳米金-链霉亲和素偶联物标记，以通过定制的银染方法增强信号。使用未经修改的常规计算机光驱进行化验读取，并使用免费的光盘质量分析软件进行数据处理。这种DVD检测的性能(灵敏度和选择性)可与成熟的比色法(光学暗度比的测定)和标准的酶联免疫吸附试验(ELISA)相媲美。通过检验其与同一组样本上的ELISA结果的线性相关性，DVD检测有望成为医生甚至家庭个人的一种低成本、多路复用的医疗点(POC)诊断工具，产生迅速的结果。
A diagnosis platform based entirely on DVD technology was developed for on-site quantitation of molecular analytes of interest, e.g., human chorionic gonadotropin (hCG) in urine samples ("quantitative pregnancy test on a disc"). An hCG-specific monoclonal antibody-binding assay prepared on a regular DVD-R was labeled with nanogold-streptavidin conjugates for signal enhancement with a customized silver-staining protocol. An unmodified, conventional computer optical drive was used for assay reading, and free disc-quality analysis software for data processing. The performance (sensitivity and selectivity) of this DVD assay is comparable to that of well-established colorimetric methods (determination of optical darkness ratios) and standard enzyme-linked immunosorbent assays (ELISA). As validated by examining its linear correlation with the ELISA results on the same set of samples, the DVD assay promises to be a low-cost, multiplex, point-of-care (POC) diagnostic tool for physicians and even for individuals at home, producing prompt results.