Cytochalasin B-induced multinucleation of murine sarcoma virus-transformed cells. Inhibition by sodium butyrate.
Cytochalasin B-induced multinucleation of murine sarcoma virus-transformed cells. Inhibition by sodium butyrate.
复制标题
细胞松弛素 B 诱导鼠肉瘤病毒转化细胞的多核化。
DOI：
10.1016/0014-4827(79)90580-9
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发表时间：
1979
影响因子：
3.7
通讯作者：
S. Steiner
中科院分区：
文献类型：
--
作者：
B. Altenburg;S. Steiner
正常大鼠肾细胞在1~3μg/ml细胞松弛素B(CB)的作用下，仅发生一次核分裂。相反，小鼠肉瘤病毒转化的NRK细胞(MSV-NRK细胞)在CB处理后出现多核。丁酸钠(2 MM)可诱导MSV-NRK细胞发生显著的形态变化，可阻止CB诱导的这些细胞的多核。二丁酰环磷酸腺苷(db-cAMP)也能恢复CB处理的MSV-NRK细胞的有限核分裂，但8-bromo-cAMP不能。在添加2 mM丁酸盐的培养液中，CB选择性地抑制MSV-NRK细胞的DNA合成，而在对照MSV-NRK细胞中则没有。这种对DNA合成的抑制可能是丁酸钠限制核分裂的原因。
Normal rat kidney (NRK) cells underwent only one nuclear division in the presence of 1–3 μg/ml cytochalasin B (CB), an inhibitor of cytoplasmic cleavage. In contrast, NRK cells transformed by murine sarcoma virus (MSV-NRK cells) became multinucleated when treated with CB. Sodium butyrate (2 mM), which induces striking morphological changes in MSV-NRK cells, prevents CB-induced multinucleation in these cells. Dibutyryl cyclic adenosine monophosphate (db-cAMP) also restores limited nuclear division to CB-treated MSV-NRK cells, but 8-bromo-cAMP does not. CB was found to selectively inhibit DNA synthesis in MSV-NRK cells grown in medium supplemented with 2 mM butyrate, but not in control MSV-NRK cells. This inhibition of DNA synthesis could account for the limitation of nuclear division by sodium butyrate.