Expression of RhoC is associated with metastasis of gastric carcinomas
Expression of RhoC is associated with metastasis of gastric carcinomas
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DOI：
10.1159/000072958
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发表时间：
2004-01-01
期刊：
影响因子：
5
通讯作者：
Yasui, W
中科院分区：
文献类型：
--
作者：
Kondo, T;Sentani, K;Yasui, W
目的：RhoC是ras相关小GTPase蛋白家族的一员，调节细胞骨架结构，并具有转化培养细胞的潜力。最近有报道称RhoC有助于黑色素瘤细胞的转移表型。本研究的目的是阐明其与胃癌发生和转移的生物学相关性。方法：采用定量RT-PCR方法检测51例术前胃癌组织（肠型24例，弥漫性27例）和8株胃癌细胞系中RhoC的表达。结果：有转移的原发肿瘤中RhoC表达水平显著高于无转移的原发肿瘤（p=0.0202； Mann-Whitney U检验）。RhoC在原发肿瘤及其转移瘤中的表达水平显著高于其对应的非肿瘤性粘膜（p分别为0.0357和0.0173；Wilcoxon符号秩检验）。胃癌细胞系中RhoC mRNA的表达得到证实。结论：我们的研究结果提示RhoC基因的表达升高可能参与胃癌的转移，并可能是预测转移潜力的良好遗传标记。版权所有(C) 2004 S. Karger AG，巴塞尔。
Objectives: RhoC, a member of the ras-related small GTPase protein family, regulates cytoskeletal structures and has the potential to transform cultured cells. It has recently been reported that RhoC contributes to the metastatic phenotype of melanoma cells. The purpose of this study was to clarify its biological relevance to gastric carcinogenesis and metastasis. Methods: We examined the expression of RhoC by quantitative RT-PCR in 51 cases of gastric carcinoma tissues from prior surgical cases (intestinal type: 24 cases, diffuse type: 27 cases) and in 8 gastric carcinoma cell lines. Results: RhoC expression levels in primary tumors were significantly higher in cases with metastasis than in those without metastasis (p=0.0202; Mann-Whitney U test). RhoC expression levels in primary tumor and their metastatic tumors were significantly higher than their corresponding nonneoplastic mucosa (p=0.0357, and 0.0173, respectively; Wilcoxon signed rank test). RhoC mRNA expression was confirmed in the gastric carcinoma cell lines. Conclusion: Our findings suggest that elevated expression of the RhoC gene may be involved in the metastasis of gastric carcinomas and may be a good genetic marker for the prediction of a metastatic potential. Copyright (C) 2004 S. Karger AG, Basel.