SOLUTION STRUCTURE OF AN UNUSUALLY STABLE RNA TETRAPLEX CONTAINING G-QUARTET AND U-QUARTET STRUCTURES
SOLUTION STRUCTURE OF AN UNUSUALLY STABLE RNA TETRAPLEX CONTAINING G-QUARTET AND U-QUARTET STRUCTURES
复制标题
DOI：
10.1021/bi00151a003
复制
发表时间：
1992-09-15
期刊：
影响因子：
2.9
通讯作者：
MOORE, PB
中科院分区：
文献类型：
--
作者：
CHEONG, CJ;MOORE, PB
用二维核磁共振谱和分子动力学方法建立了RNA四链体(RUGGGGGU)4的溶液结构模型。这种分子是平行的，在50 mM的KCl中是胡格斯汀碱基配对的，它是如此稳定，以至于它的六个亚米诺质子中的三个在40摄氏度下以天为单位交换了半衰期。四链通过碱基堆积和四个G四联体和至少一个U四联体中的氢键稳定。这是我们所知道的U-四重奏结构存在的第一个迹象。
A model for the solution structure of an RNA tetraplex, (rUGGGGU)4, has been obtained by two-dimensional NMR spectroscopy and molecular dynamics. The molecule is parallel stranded and Hoogsteen base-paired in 50 mM KCl, and it is so stable that three of its six imino protons have exchange half-lives measured in days at 40-degrees-C. The tetraplex is stabilized by base stacking and by the hydrogen bonds in four G quartets and at least one U quartet. This is the first indication of the existence of U-quartet structures of which we are aware.