A Human Tissue Culture Cell Line from a Transitional Cell Tumour of the Urinary Bladder: Growth, Chromosome Pattern and Ultrastructure
A Human Tissue Culture Cell Line from a Transitional Cell Tumour of the Urinary Bladder: Growth, Chromosome Pattern and Ultrastructure
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膀胱移行细胞肿瘤的人组织培养细胞系：生长、染色体模式和超微结构
DOI：
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发表时间：
1970
影响因子：
8.8
通讯作者：
L. M. Franks
中科院分区：
文献类型：
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作者：
C. Rigby;L. M. Franks
细胞培养物由18个人膀胱肿瘤制成。三个细胞系保持了七个转移代，但都有一个“成纤维细胞”的形态和正常的二倍体核型。第四条生产线已经维持了80多个转移世代。这是来自分化良好的膀胱乳头状肿瘤。光镜和电镜下的细胞形态结构与膀胱肿瘤相似。当移植到仓鼠的颊囊中时，这些细胞形成了具有类似结构的肿瘤结节。有一个干细胞染色体数目为48。60%的干细胞核型在C组和D组各有一条额外染色体。
Cell cultures were made from 18 human bladder tumours. Three cell lines were maintained for seven transfer generations, but all had a “fibroblastic” morphology and a normal diploid karyotype. A fourth line has been maintained for over 80 transfer generations. This was derived from a well differentiated papillary tumour of bladder. Morphologically the light and electron microscopic structure of the cells resembled that of bladder tumours. The cells formed tumour nodules, with a similar structure, when transplanted into hamster cheek pouches. There is a stem line chromosome number of 48. Karyotypes of 60% of the stem line cells had one extra chromosome in Group C and one in Group D.