Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana
Coordination of cell proliferation and cell expansion in the control of leaf size in Arabidopsis thaliana
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DOI：
10.1007/s10265-005-0232-4
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发表时间：
2006-01-01
影响因子：
2.8
通讯作者：
Tsukaya, H
中科院分区：
文献类型：
--
作者：
Horiguchi, G;Ferjani, A;Tsukaya, H
大小是表征器官形态和功能的重要参数。为了了解控制叶片大小的机制，我们先前分离了一些改变叶片大小的拟南芥突变体。由于叶片的形态发生依赖于确定的细胞增殖，所以成熟叶片的大小受细胞大小和数量的变化控制。因此，叶大小的突变体应该根据突变对细胞数量和/或大小的影响进行分类。以ANGUSTIFOLIA3/GRF相互作用因子1和aintegumenta为代表的一组突变体表现出一种有趣的细胞表型，称为补偿：当突变导致叶细胞数量减少时，叶细胞大小增加，导致叶面积补偿。一些遗传证据表明，补偿可能不是细胞分裂与细胞生长分离的结果。相反，证据表明，在叶片发育过程中，有一种器官范围的机制协调细胞增殖和细胞扩张。我们的结果提供了一个关键的、新颖的概念，解释了如何在器官水平上控制叶片大小。
Size is an important parameter in the characterization of organ morphology and function. To understand the mechanisms that control leaf size, we previously isolated a number of Arabidopsis thaliana mutants with altered leaf size. Because leaf morphogenesis depends on determinate cell proliferation, the size of a mature leaf is controlled by variation in cell size and number. Therefore, leaf-size mutants should be classified according to the effects of the mutations on the cell number and/or size. A group of mutants represented by angustifolia3/grf-interacting factor1 and aintegumenta exhibits an intriguing cellular phenotype termed compensation: when the leaf cell number is decreased due to the mutation, the leaf cell size increases, leading to compensation in leaf area. Several lines of genetic evidence suggest that compensation is probably not a result of the uncoupling of cell division from cell growth. Rather, the evidence suggests an organ-wide mechanism that coordinates cell proliferation with cell expansion during leaf development. Our results provide a key, novel concept that explains how leaf size is controlled at the organ level.