GENOME SIZE IN SPHAGNUM (PEAT MOSS)
GENOME SIZE IN SPHAGNUM (PEAT MOSS)
复制标题
泥炭藓（泥炭藓）的基因组大小
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
R. Krisai
中科院分区：
文献类型：
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作者：
Eva M. Temsch;J. Greilhuber;R. Krisai
摘要：使用基于视频的图像分析系统 (CIRES) 上进行的 Feulgen 吸光光度测定法测定了 30 种奥地利泥炭藓物种的基因组大小，并在扫描细胞光度计 (Leitz MPV II) 上进行了强相关结果的比较。使用豌豆 (1C = 4.42pg DNA) 进行内标化。在物种之间，可以明确地识别两个水平的倍性，即单倍体和二倍体（尽管严格来说，只要无法获得精确的平行染色体计数，这种识别仍然是假设的）。 26 个单倍体物种的 DNA 值从 0.392 pg 到 0.506 pg (1C)，4 个二倍体物种（包括 S. palustre 的两个品种）的 DNA 值从 0.814 pg 到 0.952 pg。水平之间的平均比率为1:2.049。区域内物种间的变异低于区域间的变异。在某些情况下，发现一个物种的种质之间存在显着差异。这里介绍的泥炭藓的基因组大小与文献中对该物种的估计存在很大偏差。
Abstract: Genome size was determined in thirty Austrian species of Sphagnum, using Feulgen absorbance photometry conducted on a video-based image analysis system (CIRES), and for comparison on a scanning cytophotometer (Leitz MPV II) with strongly correlated results. Pisum sativum (1C = 4.42pg DNA) was used for internal standardization. Between species, two levels of ploidy, haploid and diploid, could be unambiguously identified (although this identification remains, strictly speaking, hypothetical, as long as exact parallel chromosome counts are not available). Twenty-six haploid species yielded values from 0.392 pg to 0.506 pg DNA (1C), and four diploid species (including two varieties of S. palustre) from 0.814 pg to 0.952 pg. The average ratio between levels was 1:2.049. Variation between species within sections was lower than between sections. In some cases significant differences between accessions of one species were found. The genome size of Sphagnum palustre presented here strongly deviates from one estimate of this species in the literature.