Skeletal muscle and liver oxysterols during fasting and alcohol exposure
Skeletal muscle and liver oxysterols during fasting and alcohol exposure
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DOI：
10.1016/j.metabol.2005.08.003
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发表时间：
2006-01-01
影响因子：
9.8
通讯作者：
Preedy, VR
中科院分区：
文献类型：
--
作者：
Adachi, J;Kudo, R;Preedy, VR
氧固醇是具有一系列细胞作用的细胞毒性剂，包括白蛋白合成、细胞分化和细胞凋亡诱导的损伤。营养因素对它们的调节作用描述得很少。我们的目的是检验这一假设，即强制戒断食物和酒精暴露增加组织氧固醇浓度。我们测量了进食和禁食（禁食1天和2天）雄性Wistar大鼠肝脏和骨骼肌中氧固醇7 α-羟基胆甾-5-烯-3 β-醇（7 α-OH）、7 β-羟基胆甾-5-烯-3 β-醇（7 β-OH）和3 β-羟基胆甾-5-烯-7-酮（7-酮）的浓度。分析了氧化（I型;比目鱼肌）和糖酵解（II型;跖肌）肌肉。我们还研究了短期乙醇推注（处死前2.5小时IP给予75 mmol/kg体重）诱导的营养扰动剂的影响。结果表明，禁食后，肝脏、I型和II型骨骼肌中7 α-OH、7 β-OH和7-酮含量显著增加（P
Oxysterols are cytotoxic agents that have a range of cellular actions, including impairment of albumin synthesis, cell differentiation, and induction of apoptosis. Their regulations by nutritional factors are poorly described. Our objective was to test the hypothesis that the imposition of food withdrawal and alcohol exposure increases tissue oxysterol concentrations. We measured the concentrations of the oxysterols 7 alpha-hydroxycholest-5-en-3 beta-ol (7 alpha-OH), 7 beta-hydroxycholest-5-en-3 beta-ol (7 beta-OH), and 3 beta-hydroxycholest-5-en-7-one (7-keto) in liver and skeletal muscle of fed and fasted (food withdrawal for 1 and 2 days) male Wistar rats. Both oxidative (type I; soleus) and glycolytic (type II; plantaris) muscles were analyzed. We also investigated the effects of a nutritional perturbant induced by a short-term bolus of ethanol (75 mmol/kg weight IP administered 2.5 hours before sacrifice). The results showed that in response to fasting there were significant increases in 7a-OH, 7 beta-OH, and 7-keto in liver and both type I and II skeletal muscle (P