Stromal Fibroblasts Adjacent to Invasive Thyroid Tumors: Expression of Gelatinase A But Not Stromelysin 3 mRNA
Stromal Fibroblasts Adjacent to Invasive Thyroid Tumors: Expression of Gelatinase A But Not Stromelysin 3 mRNA
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邻近侵袭性甲状腺肿瘤的间质成纤维细胞：表达明胶酶 A，但不表达 Stromelysin 3 mRNA
DOI：
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发表时间：
1996
影响因子：
2.6
通讯作者：
M. Bäckdahl
中科院分区：
文献类型：
--
作者：
J. Zedenius;M. Ståhle;U. Enberg;L. Grimelius;C. Larsson;G. Wallin;M. Bäckdahl
抽象的。甲状腺肿瘤的生物学行为差异很大;它们的范围从良性腺瘤到快速生长的未分化癌。在甲状腺肿瘤中，滤泡性肿瘤特别适合作为研究肿瘤细胞浸润的模型;腺瘤和癌之间的区别主要取决于包膜和血管浸润的存在。基质金属蛋白酶能够降解基底膜和细胞外基质成分，在肿瘤细胞侵袭中起重要作用。选择29例不同类型和侵袭性的甲状腺肿瘤，通过mRNA原位杂交分析相对分子质量为72，000-道尔顿的IV型胶原酶（明胶酶A）的表达。强明胶酶A mRNA的表达被认为是在10个14滤泡癌，没有6滤泡腺瘤，在所有4个未分化癌，在4个乳头状癌。该表达仅限于邻近或接近侵袭性肿瘤细胞的间质中的成纤维细胞。还研究了12个肿瘤的基质分解素3 mRNA的表达，在任何肿瘤中均未检测到基质分解素3 mRNA的表达。研究结果表明，明胶酶A有助于侵袭性甲状腺肿瘤的侵袭过程和扩散。
Abstract. Thyroid tumors vary widely in biologic behavior; they range from benign adenomas to rapidly growing anaplastic carcinomas. Among thyroid neoplasms, the follicular tumor is especially suited as a model for studies of tumor cell invasion; the distinction between adenomas and carcinomas relies mainly on the presence of capsular and vascular invasion. Matrix metalloproteinases play an important role in tumor cell invasion, as they are able to degrade basement membrane and extracellular matrix components. Twenty-nine thyroid tumors of varying type and aggressiveness were selected for analysis of relative molecular weight 72,000-dalton type IV collagenase (gelatinase A) expression by mRNA in situ hybridization. Strong gelatinase A mRNA expression was seen in 10 of 14 follicular carcinomas, in none of six follicular adenomas, in all four anaplastic carcinomas, and in four of five papillary carcinomas. The expression was restricted to fibroblasts in the stroma adjacent or close to invading tumor cells. Twelve of the tumors were also investigated for expression of stromelysin 3 mRNA, no expression of which was detected in any tumor. The findings suggest that gelatinase A contributes to the invasive process and spread of aggressive thyroid tumors.
DOI：
10.1073/pnas.86.21.8207
发表时间：
1989-11-01
影响因子：
11.1
作者：
GOLDBERG, GI;MARMER, BL;HE, CS
通讯作者：
HE, CS
DOI：
10.1093/jnci/85.21.1758
发表时间：
1993-11-03
期刊：
JOURNAL OF THE NATIONAL CANCER INSTITUTE
影响因子：
--
作者：
AZZAM, HS;ARAND, G;THOMPSON, EW
通讯作者：
THOMPSON, EW
DOI：
10.1016/s0021-9258(18)71503-2
发表时间：
1989-10
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
W. Stetler-Stevenson;H. Krutzsch;L. Liotta
通讯作者：
W. Stetler-Stevenson;H. Krutzsch;L. Liotta