Development of a human adaptive immune system in cord blood cell-transplanted mice
Development of a human adaptive immune system in cord blood cell-transplanted mice
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DOI：
10.1126/science.1093933
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发表时间：
2004-04-02
期刊：
影响因子：
56.9
通讯作者：
Manz, MG
中科院分区：
文献类型：
--
作者：
Traggiai, E;Chicha, L;Manz, MG
由于伦理限制限制了人血淋巴系统的体内研究，因此采用了人替代小动物异种移植模型。然而，现有的模型只能维持有限的人类淋巴样细胞的发育和维持，很少产生免疫反应。本研究表明，将CD34（+）人脐带血细胞肝内注射到条件良好的新生Rag2(-/-) γ (c)（-/-）小鼠体内，可导致B细胞、T细胞和树突状细胞的新生发育；结构化的原发性和继发性淋巴器官的形成；以及功能性免疫反应的产生。这为研究人体内适应性免疫系统的发育和功能提供了一个有价值的模型。
Because ethical restrictions limit in vivo studies of the human hematolymphoid system, substitute human to small animal xenotransplantation models have been employed. Existing models, however, sustain only limited development and maintenance of human lymphoid cells and rarely produce immune responses. Here we show that intrahepatic injection of CD34(+) human cord blood cells into conditioned newborn Rag2(-/-) gamma(c)(-/-) mice leads to de novo development of B, T, and dendritic cells; formation of structured primary and secondary lymphoid organs; and production of functional immune responses. This provides a valuable model to study development and function of the human adaptive immune system in vivo.