THE CONFORMATIONAL PROPERTIES OF THE PEPTIDE-HORMONE SOMATOSTATIN (III) - ASSIGNMENT AND ANALYSIS OF THE H-1 AND C-13 HIGH-RESOLUTION NMR-SPECTRA OF SOMATOSTATIN IN AQUEOUS-SOLUTION
THE CONFORMATIONAL PROPERTIES OF THE PEPTIDE-HORMONE SOMATOSTATIN (III) - ASSIGNMENT AND ANALYSIS OF THE H-1 AND C-13 HIGH-RESOLUTION NMR-SPECTRA OF SOMATOSTATIN IN AQUEOUS-SOLUTION
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DOI：
10.1016/0014-5793(80)80995-1
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发表时间：
1980-01-01
期刊：
影响因子：
3.5
通讯作者：
DIRKX, J
中科院分区：
文献类型：
--
作者：
HALLENGA, K;VANBINST, G;DIRKX, J
肽激素生长抑素（SRIF）具有广泛的生理活性[11]，已通过合成具有受限迁移率的类似物证明其与构象特性相关[2]。生长抑素本身的构象性质迄今为止主要通过CD [3]进行研究，尽管已经通过高分辨率NMR和半经验能量计算的组合分析了一些SRIF片段[4，5]。NMR研究受到严重的谱线加宽、在215 mM下的聚集和信号分配问题的阻碍，正如人们可以容易地从含有7个ABX自旋系统的序列中看到的那样：
The peptide hormone somatostatin (SRIF) possesses a broad range of physiological activities [11, that have been shown to be related to its conformational properties by the synthesis of analogs with restricted mobility [2]. The conformational properties of somatostatin itself have until now mainly been studied by CD [3] although some SRIF fragments have been analysed by a combination of high resolution NMR and semi-empirical energy calculations [4, 5]. NMR studies have been hindered by severe line broadening, aggregation at 215 mM and by signal assignment problems as one can easily see from the sequence containing 7 ABX spin systems: