Profiling biomarkers in gingival crevicular fluid using multiplex bead immunoassay
Profiling biomarkers in gingival crevicular fluid using multiplex bead immunoassay
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DOI：
10.1016/j.archoralbio.2012.11.012
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发表时间：
2013-06-01
影响因子：
3
通讯作者：
Yoshie, Hiromasa
中科院分区：
文献类型：
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作者：
Shimada, Yasuko;Tabeta, Koichi;Yoshie, Hiromasa
目的：已有研究对牙周沟液(GCF)中的生物标志物进行研究，但由于样本量较小，测量受到限制。这项研究的目的是测定40种不同的细胞因子和趋化因子在牙周炎样本中的水平。设计：11名参加支持性牙周治疗计划的慢性牙周炎患者，其剩余探诊袋深度(PD)为>；5 mm。每个受试者都采集了一个健康部位和两个病变部位。用多重微珠免疫分析法检测了40个GCF中的生物标志物。采用实时定量聚合酶链式反应对病区牙龈卟啉单胞菌进行定量检测。结果：应用多重微球免疫分析方法，在牙周炎患者牙周组织标本中检出26种生物标志物。经Bonferroni‘s校正后，9个生物标志物的水平在病变部位和健康部位之间有显著差异。比较探查出血(BOP)阳性和BOP阴性部位病变部位26种生物标志物的水平。经多因素比较调整后，白介素1β和干扰素诱导蛋白10水平在BOP阳性病变部位显著高于BOP阴性病变部位(IL-1β，p=0.0007，IP-10；p=0.0009)。此外，牙周液中IL-1β水平与牙周血假单胞菌比例呈正相关(r=0.646，p=0.0012)。结论：牙周炎患者牙周液中IL-1β水平与牙周炎发生率、牙周血压值及牙菌斑中牙周血假单胞菌的存在密切相关。多重微珠分析在GCF研究中是有用的。这些发现有助于在牙周病的诊断中确定新的诊断方法。(C)2012爱思唯尔有限公司。保留所有权利。
Objective: Biomarkers in gingival crevicular fluid (GCF) have been investigated; however, measurements were limited by the small sample volume available. The aim of this study was to determine the levels of 40 different cytokines and chemokines in GCF samples.Design: Eleven patients with generalised chronic periodontitis participating in a supportive periodontal therapy programme with remaining probing pocket depths (PDs) of >5 mm were enrolled. One healthy and two diseased sites were sampled in each subject. Forty biomarkers in GCF were examined using a multiplex bead immunoassay. Porphyromonas gingivalis from the diseased sites was quantified by real-time polymerase chain reaction.Results: Twenty-six biomarkers were detected in the GCF samples using the multiplex bead immunoassay. The levels of nine biomarkers were significantly different between the diseased and healthy sites after adjustment with Bonferroni's correction. The level of 26 biomarkers in diseased sites was compared between bleeding on probing (BOP)-positive and BOP-negative sites. Interleukin (IL)-1 beta and interferon-inducible protein (IP)-10 levels were significantly higher in BOP-positive diseased sites than BOP-negative diseased sites after adjustment for multiple comparisons (IL-1 beta, p = 0.0007, IP-10; p = 0.0009). In addition, the levels of IL-1 beta in GCF were found to be strongly correlated with the P. gingivalis ratio (r = 0.646, p = 0.0012).Conclusion: IL-1 beta levels in GCF correlate with the PDs, BOP and the presence of P. gingivalis in subgingival plaque. Multiplex bead assays can be useful in GCF studies. These findings can help in identifying new diagnostic methods in the diagnosis of periodontal disease. (C) 2012 Elsevier Ltd. All rights reserved.