beta cell apoptosis in T cell-mediated autoimmune diabetes
beta cell apoptosis in T cell-mediated autoimmune diabetes
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DOI：
10.1073/pnas.94.1.213
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发表时间：
1997-01-07
影响因子：
11.1
通讯作者：
Katz, JD
中科院分区：
文献类型：
--
作者：
Kurrer, MO;Pakala, SV;Katz, JD
胰岛素依赖型糖尿病是由T细胞介导的对产生胰岛素的胰岛β细胞的破坏引起的。这种破坏是如何发生的仍然是难以捉摸的，主要是由于疾病进展的缓慢动力学。通过将携带β细胞特异性T细胞受体的转基因小鼠杂交到NOD上。在scid背景下，我们制作了一个简化但稳健的糖尿病加速模型。该小鼠产生携带转基因T细胞受体的CD4（+）T细胞，但缺乏CD8（+）T细胞和B细胞。更重要的是，这只老鼠迅速患上糖尿病，这使我们能够记录和量化β细胞死亡。我们已经确定炎症胰岛内的β细胞通过凋亡而死亡。
Insulin-dependent diabetes mellitus results from T cell-mediated destruction of insulin-producing, pancreatic islet beta cells. How this destruction takes place has remained elusive-largely due to the slow kinetics of disease progression. By crossing a transgenic mouse carrying a beta cell-specific T cell receptor onto the NOD. scid background, we produced a simplified but robust and accelerated model of diabetes. This mouse produces CD4(+) T cells bearing transgenic T cell receptor but is devoid of CD8(+) T cells and B cells. More importantly, this mouse develops a rapid diabetes, which has allowed us to record and quantify beta cell death. We have determined that beta cells within the inflamed islets die by apoptosis.