Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes
Species-specific identification of and distinction between Borrelia burgdorferi genomic groups by using 16S rRNA-directed oligonucleotide probes
复制标题
使用 16S rRNA 定向寡核苷酸探针对伯氏疏螺旋体基因组进行物种特异性鉴定和区分
DOI：
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发表时间：
1992
影响因子：
9.4
通讯作者：
C. F. Garon
中科院分区：
文献类型：
--
作者：
R. T. Marconi;L. Lubke;W. Hauglum;C. F. Garon
Examination of a number of previously published aligned Borrelia 16S rRNA sequences revealed the presence of regions which could serve as oligonucleotide probe targets for both species-specific identification of Borrelia burgdorferi and distinction between genomic groups. Total cellular RNA isolated from Borrelia cultures was used in slot blot analysis. Radiolabeled oligonucleotides designed to hybridize to specific 16S rRNA targets were used as probes. These probes allowed for both species-specific identification and genomic group typing of B. burgdorferi.
