Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.
Strategies to Study Dark Growth Deficient or Slower Mutants in Chlamydomonas reinhardtii.
复制标题
研究莱茵衣藻暗生长缺陷或较慢突变体的策略。
DOI：
10.1007/978-1-0716-1370-2_13
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发表时间：
2021
影响因子：
--
通讯作者：
Wenfeng Tu
中科院分区：
文献类型：
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作者：
Huanling Yang;Fei Han;Yue Wang;Wenqiang Yang;Wenfeng Tu
光合作用是地球上最重要的化学反应，约60%的CO2通过光合作用被藻类固定。由于昼夜循环，包括藻类在内的光合生物的整个生命中有一半时间处于黑暗中，并且黑暗代谢对于光合生物在再次接收光时重新开始光合作用是至关重要和必要的。简言之，暗代谢为恢复光合作用提供必要的物质和能量，再氧化NADH形成NAD+，合理储存光合产物，并维持正确的氧化还原平衡。莱茵衣藻在自养和异养条件下生长，使其成为研究光合作用、暗代谢和光暗转换的理想生物。此外，它还提供了一个很好的模型，以确定关键的分子组成和阐明异养的分子调控机制，这为理解光合生物如何从黑暗中重新启动光合作用提供了新的线索。暗生长缺陷型或生长缓慢型的衣原体突变体可能是由于乙酸的吸收和运输效率低下、线粒体电子传递链蛋白质受损、线粒体功能障碍、暗条件下氧化还原状态改变或线粒体与其他细胞器之间的通讯障碍、氧化还原失衡或光合产物分布紊乱等原因造成的。本文就莱茵衣藻突变体的分析方法和策略进行综述。
Photosynthesis is the most important chemical reaction on the earth, and about 60% of the CO2 is fixed by algae through photosynthesis. Photosynthetic organisms including algae experience half of the entire life in the dark due to diel cycles, and dark metabolism is critical and necessary for photosynthetic organisms to restart photosynthesis when receiving light again. Briefly, dark metabolism provides necessary materials and energy for restoring photosynthesis, reoxidizes NADH to form NAD+, rationally stores photosynthates, and maintains correct redox balance. Chlamydomonas reinhardtii grows under both autotrophic and heterotrophic conditions, making it an ideal organism to study photosynthesis, dark metabolism, and light dark transitions as well. In addition, it provides a good model to identify key molecular components and elucidate the molecular regulatory mechanisms of heterotrophic, which provides new clues to understand how photosynthetic organisms restart photosynthesis from the dark. Chlamydomonas mutants with dark growth deficiency or slower growth phenotypes are likely caused by the inefficient uptake and transport of acetate, the damaged proteins of mitochondrial electron transport chain, the malfunctioned mitochondrion, the redox state alteration in the dark or failed communication between mitochondrion and other organelles, the imbalanced redox or the disrupted distribution of the photosynthetic products. Here we summarize the methods and strategies for analyzing these mutants in Chlamydomonas reinhardtii.