A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples
A simple, rapid and high-throughput fluorescence polarization immunoassay for simultaneous detection of organophosphorus pesticides in vegetable and environmental water samples
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一种简单、快速、高通量的荧光偏振免疫分析方法，可同时检测蔬菜和环境水样中的有机磷农药
DOI：
10.1016/j.aca.2011.09.040
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发表时间：
2011-12-05
影响因子：
6.2
通讯作者：
Sun, Yuan-Ming
中科院分区：
文献类型：
--
作者：
Xu, Zhen-Lin;Wang, Qiang;Sun, Yuan-Ming
建立了一种简便、快速、高通量的单克隆抗体荧光偏振免疫分析法（FPIA），用于有机磷农药的同时检测。研究了示踪剂结构、示踪剂浓度、抗体稀释度、甲醇含量和基质效应对荧光偏振免疫分析性能的影响。该方法可同时检测5种有机磷农药，检测限低于10 ng/mL。抗体-抗原相互作用的平衡所需的时间小于10分钟。从蔬菜和环境样品的加标回收率为71.3%至126.8%，变异系数为3.5%至14.5%。所开发的FPIA应用于样品，然后与高效液相色谱-串联质谱（HPLC-MS/MS）分析确认。该方法具有良好的准确性和重现性，适用于OP污染的快速、高通量筛查，且效率高、成本低。(C)2011爱思唯尔有限公司版权所有。
A simple, rapid and high-throughput fluorescent polarization immunoassay (FPIA) for simultaneous determination of organophosphorus pesticides (OPs) using a broad-specificity monoclonal antibody was developed. The effects of tracer structure, tracer concentration, antibody dilution, methanol content and matrix effect on FPIA performance were studied. The FPIA can detect 5 OPs simultaneously with a limit of detection below 10 ng mL(-1). The time required for the equilibrium of antibody-antigen interaction was less than 10 min. The recovery from spiked vegetable and environmental samples ranged from 71.3% to 126.8%, with the coefficient of variations ranging from 3.5% to 14.5%. The developed FPIA was applied to samples, followed by confirmation with high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) analysis. The developed FPIA demonstrated good accuracy and reproducibility, and is suitable for rapid and high-throughput screening for OP contamination with high-efficiency and low cost. (C) 2011 Elsevier B.V. All rights reserved.