A Transgenic mouse Class-III β tubulin reporter using yellow fluorescent protein
A Transgenic mouse Class-III β tubulin reporter using yellow fluorescent protein
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DOI：
10.1002/dvg.20325
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发表时间：
2007-09-01
期刊：
影响因子：
1.5
通讯作者：
Goldowitz, Dan
中科院分区：
文献类型：
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作者：
Li Liu;Geisert, Eldon E.;Goldowitz, Dan
将黄色荧光蛋白 (YFP) 报告基因构建体克隆到 P-微管蛋白 III 启动子的下游，并注射以产生两个转基因小鼠的创始品系。在发育中的外周和中枢神经系统的许多区域中观察到 YFP 表达。早在胚胎第 9 天，YFP 表达就首次在外周和中枢神经系统中观察到。通过 PO 表达 YFP 的神经元系统数量急剧增加。然后，随着动物达到成年年龄，表达水平下降，但许多神经元仍然表现出 YFP 表达，特别是在正在经历成年神经发生的大脑区域，即吻侧迁移流和齿状回的颗粒下层。将这种基于报告基因的染色与抗 III 类 P-微管蛋白免疫细胞化学进行比较，结果显示与内源蛋白的表达非常相似。这些转基因系应该提供独特的模型来研究体内和体外神经发育。
A yellow fluorescence protein (YFP) reporter construct was cloned downstream of the P-tubulin III promoter and injected to produce two founder lines of transgenic mice. YFP expression was observed in many regions of the developing peripheral and central nervous system. YFP expression was first observed in the peripheral and central nervous system as early as embryonic day 9.0. There was a dramatic increase in the number of neuronal systems expressing YFP through PO. Then as the animals reached adult age, the expression levels decreased, but many neurons still show YFP expression, notably in regions of the brain undergoing adult neurogenesis, i.e., the rostral migratory stream and subgranular layer of the dentate gyrus. This reporter-based staining was compared with anti-class-III P-tubulin immunocytochemistry and shown to closely parallel the expression of the endogenous protein. These transgenic lines should provide unique models to study in vivo and in vitro neurodevelopment.