Quantitative leukoviremia and immune complex-dissociated antigenemia as predictors of infection status in children born to mothers infected with human immunodeficiency virus type 1.
Quantitative leukoviremia and immune complex-dissociated antigenemia as predictors of infection status in children born to mothers infected with human immunodeficiency virus type 1.
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定量白病毒血症和免疫复合物解离抗原血症是感染 1 型人类免疫缺陷病毒的母亲所生儿童感染状态的预测因子。
DOI：
10.1016/s0022-3476(05)83530-9
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Miller,G
中科院分区：
文献类型：
--
作者：
Ikeda,MK;Andiman,WA;Mezger,JL;Shapiro,ED;Miller,G
从外周血单个核细胞培养人类免疫缺陷病毒1型（HIV-1）的四种方法和两种血清抗原试验被评估为HIV-1感染母亲所生儿童感染状况的预测指标。共培养的36名婴儿中，根据临床标准或Western blot反应性抗体的持久性认为感染HIV-1的15名婴儿（9名有症状，6名无症状）的培养结果均为阳性，21名未感染的血清转化者的培养结果均为阴性（敏感性=100%，特异性=100%）。定量共培养比用植物血凝素计数和刺激细胞但不与血清阴性供者的细胞共培养的技术更敏感，比在80名儿童样本中评估的另外两种定性技术更敏感，其中细胞在培养前未计数。有症状儿童的白细胞病毒血症水平与无症状感染儿童的白细胞病毒血症水平没有明显差异。在定量共培养阳性的患者中，只有40%的患者血清中有游离HIV-1抗原，而86%的患者血清中有免疫复合物中的抗原。在评估的方法中，定量HIV-1共培养是儿童感染状况的最佳指标。
Four methods of culturing human immunodeficiency virus type 1 (HIV-1) from peripheral blood mononuclear cells and two serum antigen tests were assessed as predictors of infection status in children born to HIV-1-infected mothers. Of 36 infants whose cocultures were quantitative, all 15 who were deemed to be infected with HIV-1 (nine with symptoms, six without symptoms) by clinical criteria or persistence of Western blot reactive antibody had positive culture results, and all 21 uninfected seroreverters had negative culture results (sensitivity=100%; specificity=100%). Quantitative coculture was more sensitive than a technique in which cells were counted and stimulated with phytohemagglutinin but not cocultivated with cells from seronegative donors, and more sensitive than two other qualitative techniques evaluated in samples from 80 children, in which cells were not enumerated before culture. The level of leukoviremia in children with symptoms did not differ appreciably from the level of leukoviremia in symptom-free infected children. Among those with positive results on quantitative coculture, only 40% also had free HIV-1 antigen in serum, whereas 86% had antigen in immune complexes. Among the methods evaluated, quantitative HIV-1 coculture was the best indicator of infection status in children.