Propagation characteristics of laser-induced stress wave in deep tissue for gene transfer
Propagation characteristics of laser-induced stress wave in deep tissue for gene transfer
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DOI：
10.1016/j.apsusc.2009.04.115
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发表时间：
2009-09-30
影响因子：
6.7
通讯作者：
Obara, Minoru
中科院分区：
文献类型：
--
作者：
Ando, Takahiro;Sato, Shunichi;Obara, Minoru
研究了激光诱导应力波（LISW）在组织中的传播特性及其与基因转染特性的相关性。LISW产生的激光吸收材料与532 nm的Q开关Nd：YAG激光脉冲照射的透明塑料片附着在等离子体约束的吸收材料。用针型水听器测量了LISW在不同厚度组织中传播的时间压力分布，并用阴影技术观察了LISW在水中的传播。测量结果表明，在激光能量密度为1.2 J/cm（2），激光光斑直径为3 mm时，在高达5 mm的深度内保持平坦波前，峰值压力P随着组织厚度d的增加而降低; P与d（0.54）成正比。将编码报告基因的质粒DNA注入大鼠背部皮肤，在其上放置不同数量的离体皮肤，并从皮肤顶部施加LISW。在3 mm厚的堆叠皮肤下的皮肤中观察到有效的基因表达，表明可以通过经皮应用LISW来转染深部组织如肌肉。(C)2009爱思唯尔有限公司版权所有。
Propagation characteristics of laser-induced stress waves (LISWs) in tissue and their correlation with properties of gene transfection were investigated for targeted deep-tissue gene therapy. LISWs were generated by irradiating a laser-absorbing material with 532-nm Q-switched Nd:YAG laser pulses; a transparent plastic sheet was attached on the absorbing material for plasma confinement. Temporal pressure profiles of LISWs that were propagated through different thickness tissues were measured with a needle-type hydrophone and propagation of LISWs in water was visualized by shadowgraph technique. The measurements showed that at a laser fluence of 1.2 J/cm(2) with a laser spot diameter of 3 mm, flat wavefront was maintained for up to 5 mm in depth and peak pressure P decreased with increasing tissue thickness d; P was proportional to d (0.54). Rat dorsal skin was injected with plasmid DNA coding for reporter gene, on which different numbers of excised skin(s) was/were placed, and LISWs were applied from the top of the skins. Efficient gene expression was observed in the skin under the 3 mm thick stacked skins, suggesting that deep-located tissue such as muscle can be transfected by transcutaneous application of LISWs. (C) 2009 Elsevier B.V. All rights reserved.