The Zinc-Responsive Regulator Zur Controls Expression of the Coelibactin Gene Cluster in Streptomyces coelicolor
The Zinc-Responsive Regulator Zur Controls Expression of the Coelibactin Gene Cluster in Streptomyces coelicolor
复制标题
DOI：
10.1128/jb.01022-09
复制
发表时间：
2010-01-01
影响因子：
3.2
通讯作者：
Paget, Mark S. B.
中科院分区：
文献类型：
--
作者：
Kallifidas, Dimitris;Pascoe, Ben;Paget, Mark S. B.
缺乏锌反应的Zur阻遏物的天蓝色链霉菌突变株在产孢子过程中有条件缺陷，可能是由于一个或多个Zur靶基因的过度表达所致。基因中断分析表明，先前已知的Zur靶标的解除调控并不是产孢表型的原因。我们使用微阵列来识别更多的Zur靶标，并发现Zur控制着一组基因，预计这些基因将指导合成一种名为Coelibactin的未表征的铁载体相关非核糖体编码肽。一个关键的Coelibactin生物合成基因的中断抑制了Zur产孢表型，这表明Coelibactin合成的放松调控抑制了产孢量。
Streptomyces coelicolor mutants lacking the zinc-responsive Zur repressor are conditionally defective in sporulation, presumably due to the overexpression of one or more Zur target genes. Gene disruption analyses revealed that deregulation of previously known Zur targets was not responsible for the sporulation phenotype. We used microarrays to identify further Zur targets and discovered that Zur controls a cluster of genes predicted to direct synthesis of an uncharacterized siderophore-related non-ribosomally encoded peptide designated coelibactin. Disruption of a key coelibactin biosynthetic gene suppressed the Zur sporulation phenotype, suggesting that deregulation of coelibactin synthesis inhibits sporulation.