CYCLIN-A IS REQUIRED FOR THE ONSET OF DNA-REPLICATION IN MAMMALIAN FIBROBLASTS
CYCLIN-A IS REQUIRED FOR THE ONSET OF DNA-REPLICATION IN MAMMALIAN FIBROBLASTS
复制标题
DOI：
10.1016/0092-8674(91)90293-8
复制
发表时间：
1991-12-20
期刊：
影响因子：
64.5
通讯作者：
LAMB, NJC
中科院分区：
文献类型：
--
作者：
GIRARD, F;STRAUSFELD, U;LAMB, NJC
细胞周期蛋白A蛋白在非转化哺乳动物成纤维细胞S期开始时合成并定位于细胞核中。在G1期，通过显微注射编码反义细胞周期蛋白A cDNA或亲和纯化的抗细胞周期蛋白A抗体的质粒来抑制细胞周期蛋白A的合成或活性，可以消除质粒注射细胞中细胞周期蛋白A的核染色，这两种方法都导致DNA合成的抑制。注射包括反义细胞周期蛋白B在内的其他反义载体没有观察到类似的效果，并且将纯化的人细胞周期蛋白A蛋白再注射到细胞周期蛋白A反义注射的细胞中有效地减轻了这种DNA合成的抑制。总之，这些数据表明，细胞周期蛋白A在哺乳动物细胞中的DNA复制的控制中起着重要作用。
Cyclin A protein is synthesized and localized into the nucleus at the onset of S phase in nontransformed mammalian fibroblasts. Inhibition of cyclin A synthesis or activity through microinjection of plasmids encoding antisense cyclin A cDNA or affinity-purified anti-cyclin A antibodies during G1 phase was shown to abolish the nuclear staining for cyclin A in plasmid-injected cells, and both procedures led to inhibition of DNA synthesis. No similar effect was observed with injection of other antisense vectors including antisense cyclin B, and reinjection of purified human cyclin A protein into cyclin A antisense-injected cells effectively relieved this inhibition of DNA synthesis. Taken together, these data suggest that cyclin A plays a major role in the control of DNA replication in mammalian cells.