GERMLINE TRANSFORMATION USED TO DEFINE KEY FEATURES OF HEAT-SHOCK RESPONSE ELEMENTS
GERMLINE TRANSFORMATION USED TO DEFINE KEY FEATURES OF HEAT-SHOCK RESPONSE ELEMENTS
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DOI：
10.1126/science.3125608
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发表时间：
1988-03-04
期刊：
影响因子：
56.9
通讯作者：
LIS, JT
中科院分区：
文献类型：
--
作者：
XIAO, H;LIS, JT
热休克共有元件 (HSE) CTNGAANNTTCNAG 存在于所有热休克基因上游的多个拷贝中。在此，通过将 hsp70-lacZ 基因与一对合成 HSE 融合的果蝇种系转化来测试热激诱导的序列要求。任一 HSE 中的某些单碱基取代都会导致表达急剧减少（四十倍）。令人惊讶的是，紧邻 HSE 两侧的序列变化也降低了诱导水平。一种这样的变体包含两个完美的 14 碱基对 HSE，它们之间和 TATA 盒之间的间隔正确，仅保留了野生型诱导表达的 7%。这些和其他分析表明，热休克调节元件包括 14 碱基对 HSE 之外的序列，并且可以更好地描述为 10 碱基对序列 NTTCNNGAAN 的二聚体。
The heat-shock consensus element (HSE), CTNGAANNTTCNAG, is found in multiple copies upstream of all heat-shock genes. Here, the sequence requirements for heat-shock induction are tested by Drosophila germline transformation with an hsp70-lacZ gene fused to a pair of synthetic HSEs. Certain single-base substitutions in either HSE cause a dramatic reduction (fortyfold) in expression. Surprisingly, variations in sequences immediately flanking the HSEs also reduced levels of induction. One such variant that contains two perfect 14-base pair HSE, which are correctly spaced relative to each other and the TATA box, retained only 7% of wild type-induced expression. These and additional analyses indicate that the heat-shock regulatory element includes sequences beyond the 14-base pair HSE and may be better described as a dimer of a 10-base pair sequence, NTTCNNGAAN.