Bidirectional activity-dependent morphological plasticity in hippocampal neurons
Bidirectional activity-dependent morphological plasticity in hippocampal neurons
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DOI：
10.1016/j.neuron.2004.11.016
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发表时间：
2004-12-02
期刊：
影响因子：
16.2
通讯作者：
Bonhoeffer, T
中科院分区：
文献类型：
--
作者：
Nägerl, UV;Eberhorn, N;Bonhoeffer, T
锥体神经元上的树突棘接收绝大多数兴奋性输入，并且被认为是电生化处理单元，整合和划分突触输入。在突触可塑性之后，棘可以经历形态可塑性，这可能形成神经元回路长期变化的结构基础。在这里，我们表明，从器官型切片培养的CA 1锥体神经元的棘显示双向活动依赖的形态可塑性。使用双光子延时显微镜，我们观察到，低频刺激诱导NMDA受体依赖性的脊柱回缩，而θ爆发刺激导致新的脊柱的形成。此外，在没有刺激的情况下，脊柱回缩的数量与刺激诱导的脊柱增益或损失处于相同的数量级。最后，我们发现神经元以活动依赖的方式消除棘的能力随着发育年龄的增长而下降。总之，我们的数据表明，海马神经元可以进行双向形态可塑性;棘的形成和消除的活动依赖性的方式。
Dendritic spines on pyramidal neurons receive the vast majority of excitatory input and are considered electrobiochemical processing units, integrating and compartmentalizing synaptic input. Following synaptic plasticity, spines can undergo morphological plasticity, which possibly forms the structural basis for long-term changes in neuronal circuitry. Here, we demonstrate that spines on CA1 pyramidal neurons from organotypic slice cultures show bidirectional activity-dependent morphological plasticity. Using two-photon time-lapse microscopy, we observed that low-frequency stimulation induced NMDA receptor-dependent spine retractions, whereas theta burst stimulation led to the formation of new spines. Moreover, without stimulation the number of spine retractions was on the same order of magnitude as the stimulus-induced spine gain or loss. Finally, we found that the ability of neurons to eliminate spines in an activity-dependent manner decreased with developmental age. Taken together, our data show that hippocampal neurons can undergo bidirectional morphological plasticity; spines are formed and eliminated in an activity-dependent way.