Comparative analysis of induction pattern of programmed cell death and defense-related responses during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant
Comparative analysis of induction pattern of programmed cell death and defense-related responses during hypersensitive cell death and development of bacterial necrotic leaf spots in eggplant
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DOI：
10.1007/s00425-006-0277-1
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发表时间：
2006-10-01
期刊：
影响因子：
4.3
通讯作者：
Hikichi, Yasufumi
中科院分区：
文献类型：
--
作者：
Kiba, Akinori;Takata, Osamu;Hikichi, Yasufumi
池鸡假单胞菌引起坏死性叶斑（NLS），而丁香假单胞菌引起叶斑坏死。烟草可引起茄子的超敏反应（HR）。绦虫在接种后9 h诱导细胞死亡（HAI），在24-30 HAI左右达到最大值。另一方面，丁香假单胞菌可诱导细胞死亡。烟叶，最高可达12-18 HAI左右。两种细菌接种茄子均产生超氧化物。接种这两种细菌也能诱导DNA断裂、细胞色素c释放到细胞质中以及PR-1和hsr203J等防御相关基因的表达，但这些植物反应在接种紫丁香假单胞菌的茄子中被诱导得更快。而不是有恙螨的。接种丁香假单胞菌后，茄子体内脂质过氧化和脂氧合酶（LOX）的诱导作用明显增强。与接种了滴虫的茄子相比。药理学研究表明，这两种细菌诱导的细胞死亡、NLS或HR通常与新生蛋白合成、活性氧和caspase iii样蛋白酶有关。有趣的是，脂质过氧化、LOX、丝氨酸蛋白酶和dna酶的参与在NLS和HR的诱导中有所不同。这些结果表明，细胞程序性死亡可能不仅与HR密切相关，而且与NLS密切相关。然而，HR和NLS不仅在诱导动力学和植物反应水平上存在差异，而且在侵染相关反应上也存在差异。
Pseudomonas cichorii causes necrotic leaf spots (NLS), while Pseudomonas syringae pv. tabaci induces a hypersensitive response (HR) in eggplant. P. cichorii induced cell death at 9 h after inoculation (HAI), reaching a maximum of around 24-30 HAI. On the other hand, cell death was induced 6 HAI with P. syringae pv. tabaci, reaching a maximum of around 12-18 HAI. Superoxide generation was observed in eggplant inoculated with both bacteria. DNA fragmentation, cytochrome c release into the cytosol and expression of defense-related genes such as PR-1 and hsr203J was also induced by inoculation with both bacteria, but these plant reactions were more rapidly induced in eggplant inoculated with P. syringae pv. tabaci rather than those with P. cichorii. Lipid peroxidation and induction of lipoxygenase (LOX) was drastically induced in eggplant inoculated with P. syringae pv. tabaci compared to P. cichorii-inoculated eggplant. Pharmacological studies showed that induction of the cell death, and the NLS or the HR in response to both bacteria was commonly associated with de novo protein synthesis, reactive oxygen species and caspase III-like protease. Interestingly, involvement of lipid peroxidation, LOX, serine protease, and DNase differed between induction of NLS and HR. These results suggest that programmed cell death might be closely associated not only with the HR but also NLS. However, there may be differences not only in the induction kinetics and level of plant responses but also in the infection-related responses between HR and NLS.