Field programming to achieve uniform sensitivity for on-line detection in electrophoresis.
Field programming to achieve uniform sensitivity for on-line detection in electrophoresis.
复制标题
现场编程以实现电泳在线检测的统一灵敏度。
DOI：
10.1002/elps.1150150138
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
Zare,RN
中科院分区：
文献类型：
--
作者：
Shear,JB;Dadoo,R;Zare,RN
Under constant applied electric field, an analyte band traverses an on‐line detection zone in an electrophoretic separation at a velocity inversely proportional to the detection time. Because analyte bands migrate with different velocities, the sensitivity of the detection system to different species can vary significantly. One approach for overcoming this problem is to change the applied electric field,E, as a function of the separation time, τ, so that the velocity of an analyte band when it passes through the detection zone is independent of the analyte's electrophoretic mobility. A possible solution is to makeE(τ) grow exponentially, but this procedure causes band compression. The preferred solution is to make bothE(τ) and the distance between the starting position of the sample and the detection zone increase linearly with separation time. This approach can be implemented with either slab‐gel or capillary electrophoresis.
