Initial in vitro interaction of osteoblasts with nano-porous alumina
Initial in vitro interaction of osteoblasts with nano-porous alumina
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DOI：
10.1016/s0142-9612(03)00146-7
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发表时间：
2003-08-01
期刊：
影响因子：
14
通讯作者：
Di Silvio, L
中科院分区：
文献类型：
--
作者：
Karlsson, M;Pålsgård, E;Di Silvio, L
在目前的研究中，我们使用了一个特色化的原代人类细胞培养模型来研究细胞与纳米多孔氧化铝的相互作用。这种材料是通过阳极氧化制备的，正在被开发为钛合金植入物的涂层。X-射线衍射仪和透射电子显微镜表明氧化铝的结构为无定形。在研究细胞/材料相互作用时，我们同时使用了生化参数和形态参数。通过细胞氧化还原反应、细胞DNA、氚胸腺嘧啶核苷([H-3]-TdR)掺入和碱性磷酸酶(ALP)生成来评价细胞存活率、增殖和表型。结果显示，在最初的2周内，随着细胞数量的增加，成骨细胞的生长模式正常。细胞增殖高峰出现在第3天，之后细胞生长减慢，碱性磷酸酶生成增加，表明成骨细胞表型在材料表面保持不变。细胞黏附，成骨样细胞形态扁平，丝状细胞附着在材料的孔上。SDS-PAGE和Western印迹分析表明，纳米多孔氧化铝能够吸附纤维连接蛋白。(C)2003爱思唯尔科学有限公司。保留所有权利。
In the present study we have used a characterised primary human cell culture model to investigate cellular interactions with nano-porous alumina. This material, prepared by anodisation, is being developed as a coating on titanium alloy implants. The structure of the alumina, as determined by X-ray diffraction and transmission electron microscopy, was amorphous.When studying cell/material interactions we used both biochemical and morphological parameters. Cell viability, proliferation and phenotype were assessed by measurement of redox reactions in the cells, cellular DNA, tritiated thymidine ([H-3]-TdR) incorporation and alkaline phosphatase (ALP) production. Results showed a normal osteoblastic growth pattern with increasing cell numbers during the first 2 weeks. A peak in cell proliferation was seen on day 3, after which cell growth decreased, followed by an increase in ALP production, thus indicating that the osteoblastic phenotype was retained on the alumina.Cell adhesion was observed, the osteoblast-like cells having a flattened morphology with filipodia attached to the pores of the material.SDS-PAGE and western blot measurements showed that the nano-porous alumina was able to adsorb fibronectin.Trace amounts of aluminium ions were measured in the surrounding medium, but no adverse effect on cell activity was observed. (C) 2003 Elsevier Science Ltd. All rights reserved.