Oligonucleotides comprised of alternating 2′-deoxy-2′-fluoro-β-D-arabinonucleosides and D-2′-deoxyribonucleosides (2′F-ANA/DNA 'altimers') induce efficient RNA cleavage mediated by RNase H
Oligonucleotides comprised of alternating 2′-deoxy-2′-fluoro-β-D-arabinonucleosides and D-2′-deoxyribonucleosides (2′F-ANA/DNA 'altimers') induce efficient RNA cleavage mediated by RNase H
复制标题
DOI：
10.1016/s0960-894x(02)00439-0
复制
发表时间：
2002-09-16
影响因子：
2.7
通讯作者：
Damha, MJ
中科院分区：
文献类型：
--
作者：
Min, KL;Viazovkina, E;Damha, MJ
合成了由2 '-脱氧-2'-氟-D-阿拉伯糖核酸（2 'F-ANA）和DNA的交替残基组成的嵌合寡核苷酸，并评价了其重要的反义性质-引起核糖核酸酶H（RNase H）降解互补RNA的能力。实验使用人RNase HII和大肠杆菌RNase HI。包含交替的2 'F-ANA的三个核苷酸片段和DNA的三个核苷酸片段的混合骨架寡聚物在引发靶RNA的RNase H降解方面是最有效的，并且显著优于完全由DNA组成的寡核苷酸，这表明这些混合骨架寡核苷酸可能是有效的反义剂。(C)2002爱思唯尔科技有限公司。保留所有权利。
Chimeric oligonucleotides comprised of alternating residues of 2'-deoxy-2'-fluoro-D-arabinonucleic acid (2'F-ANA) and DNA were synthesized and evaluated for an important antisense property-the ability to elicit ribonuclease H (RNase H) degradation of complementary RNA. Experiments used both human RNase HII and Escherichia coli RNase HI. Mixed backbone oligomers comprising alternating three-nucleotide segments of 2'F-ANA and three-nucleotide segments of DNA were the most efficient at eliciting RNase H degradation of target RNA, and were significantly better than oligonucleotides entirely composed of DNA, suggesting that these mixed backbone oligonucleotides may be potent antisense agents. (C) 2002 Elsevier Science Ltd. All rights reserved.