Activation of single whisker barrel in rat brain localized by functional magnetic resonance imaging
Activation of single whisker barrel in rat brain localized by functional magnetic resonance imaging
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DOI：
10.1073/pnas.93.1.475
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发表时间：
1996-01-09
影响因子：
11.1
通讯作者：
Shulman, RG
中科院分区：
文献类型：
--
作者：
Yang, XJ;Hyder, F;Shulman, RG
先前在皮质第四层中建立的大鼠胡须的皮质表示包含不同的细胞聚集体圆柱，这些细胞聚集体被称为桶，并与对侧大鼠面部的胡须以一对一的关系相关联。在本研究中，使用大鼠大脑的功能磁共振成像 (fMRI) 来绘制单个/多个胡须机械上下运动（8 Hz 时+/-2.5 毫米振幅）期间胡须桶激活的图谱。多层梯度回波功能磁共振成像实验在 7 T 下进行，面内图像分辨率为 220 x 220 μm，切片厚度为 1 mm，回波时间为 16 ms，在刺激单个/多个晶须时观察到高度显着（P < 0.001）和局部对侧激活区域。在所有实验中（n = 10），相对于前囟和中线的激活位置与相应胡须桶的神经解剖位置高度相关，并且结果在动物内和动物间是可重复的。我们的结果表明，基于血氧水平依赖性图像对比度的功能磁共振成像能够敏感地描述大鼠大脑中单个胡须桶的激活。这种非侵入性技术将补充大鼠桶状皮层研究中的现有方法，并且对于同一动物中枢神经系统的长期研究特别有用。
The previously established cortical representation of rat whiskers in layer IV of the cortex contains distinct cylindrical columns of cellular aggregates, which are termed barrels and correlate in a one-to-one relation to whiskers on the contralateral rat face. In the present study, functional magnetic resonance imaging (fMRI) of the rat brain was used to map whisker barrel activation during mechanical up-down movement (+/-2.5 mm amplitude at 8 Hz) of single/multiple whisker(s). Multislice gradient echo fMRI experiments were performed at 7 T with in-plane image resolution of 220 x 220 mu m, slice thickness of 1 mm, and echo time of 16 ms, Highly significant (P < 0.001) and localized contralateral regions of activation were observed upon stimulation of single/multiple whisker(s). In all experiments (n = 10), the locations of activation relative to bregma and midline were highly correlated with the neuroanatomical position of the corresponding whisker barrels, and the results were reproducible intra- and interanimal. Our results indicate that fMRI based on blood oxygenation level-dependent image contrast has the sensitivity to depict activation of a single whisker barrel in the rat brain. This noninvasive technique will supplement existing methods in the study of rat barrel cortex and should be particularly useful for the long-term investigations of central nervous system in the same animal.