Uptake of a fluorescent deoxyglucose analog (2-NBDG) in tumor cells
Uptake of a fluorescent deoxyglucose analog (2-NBDG) in tumor cells
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DOI：
10.1007/s11307-005-0011-6
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发表时间：
2005-11-01
影响因子：
3.1
通讯作者：
Mullani, N
中科院分区：
文献类型：
--
作者：
O'Neil, RG;Wu, L;Mullani, N
目的：最近由Yoshioka K，Takahashi H，Homma T，Sato M，Ki Bong O，Nemoto Y，Matsuoka H（1996）A novel fluorescent derivative of glucose applied to the assessment of glucose uptake activity of Escherichia coli. Biochim Biophys Acta 1289：5-9]，并显示被转运到正常细胞中。本初步研究的目的是评估使用这种荧光2-脱氧葡萄糖类似物，2-[N-（7-硝基苯-2-氧杂-1，3-二唑-4-基）氨基]-2-脱氧葡萄糖（2-NBDG），作为一个敏感的探针，用于监测葡萄糖摄取到恶性肿瘤cellect.Procedures：MCF-7乳腺癌上皮细胞生长，并铺在盖玻片上，通过荧光成像显微镜分析2-NBDG摄取。结果如下：2-NBDG摄取的稳态荧光分析显示，前1 - 5分钟摄取迅速，然后减慢，在20-30分钟附近达到表观最大摄取。向培养基中添加5 MM D-葡萄糖显著降低2-NBDG摄取。非恶性上皮细胞（M-1上皮细胞）的2-NBDG摄取缓慢，平均小于20%的观察致瘤细胞，MCF-7乳腺癌细胞和HepG 2肝癌细胞line.Conclusions：初步数据清楚地表明，2-NBDG快速摄取到肿瘤细胞，可以通过荧光成像分析监测。摄取显示饱和和与D-葡萄糖竞争，所有性质预期2-NBDG摄取和保留在癌细胞中。其他研究，包括其他恶性细胞系和对照细胞之间的比较，将需要充分表征2-NBDG摄取的动力学特性和这种2-DG类似物作为恶性细胞中葡萄糖摄取的探针的潜在用途。
Purpose: A new fluorescent analog of D-glucose was recently developed by [Yoshioka K, Takahashi H, Homma T, Sato M, Ki Bong O, Nemoto Y, Matsuoka H (1996) A novel fluorescent derivative of glucose applicable to the assessment of glucose uptake activity of Escherichia coli. Biochim Biophys Acta 1289:5-9] and shown to be transported into normal cells. The purpose of this preliminary study was to assess the use of this fluorescent 2-deoxyglucose analog, 2-[N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl)amino]-2-deoxyglucose (2-NBDG), as a sensitive probe for monitoring glucose uptake into malignant tumor cells.Procedures: MCF-7 breast cancer epithelial cells were grown and plated on coverslips for analysis of 2-NBDG uptake via fluorescence imaging microscopy. Results: Steady-state fluorescence analysis of 2-NBDG uptake displayed rapid uptake for the first one to five minutes, then slowed, reaching an apparent maximum uptake near 20-30 minutes. Addition of 5 MM D-glucose to the media markedly reduced 2-NBDG uptake. Uptake of 2-NBDG in nonmalignant epithelial cells (M-1 epithelial cells) was slow, averaging less than 20% of that observed for tumorigenic cells, the MCF-7 breast cancer cells and the HepG2 liver cancer cell line.Conclusions: The preliminary data clearly demonstrate a rapid uptake of 2-NBDG into tumor cells that can be monitored by fluorescence imaging analysis. The uptake displays saturation and competition with D-glucose, all properties expected for 2-NBDG uptake and retention in cancer cells. Additional studies, including comparisons among other malignant cell lines and control cells, will be needed to fully characterize the kinetic properties of 2-NBDG uptake and the potential use of this 2-DG analog as a probe for glucose uptake in malignant cells.