Discrimination of peptides by using a molecularly imprinted piezoelectric biosensor
Discrimination of peptides by using a molecularly imprinted piezoelectric biosensor
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DOI：
10.1002/chem.200304952
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发表时间：
2003-10-17
影响因子：
4.3
通讯作者：
Wu, TZ
中科院分区：
文献类型：
--
作者：
Lin, CY;Tai, DF;Wu, TZ
基于在金电极上直接形成分子印迹聚合物，我们研制了一种用于检测小分子肽的肽传感器。采用一种新的交联单体（N-Acr-L-Cys-NHBn）（2）将其固定在芯片表面并与其他单体共聚。有趣的是，N-苄基丙烯酰胺参与聚合和识别是在水环境中进行的。通过使用石英晶体微天平检测，短肽可以通过它们与针对靶肽的特异性塑料抗体的相互作用来监测。分子印迹聚合物的选择性和这种人工生物传感器的灵敏度相结合，以区分痕量的催产素和加压素的ng mL（-1）规模。
Based on the direct formation of a molecularly imprinted polymer on gold electrodes, we have developed a peptide sensor for the detection of low-molecular-weight peptides. A new cross-linking monomer, (N-Acr-L-Cys-NHBn)(2), was employed to attach the surface of the chip and to copolymerize with other monomers. Interestingly, N-benzylacrylamide participates in the polymerization and recognition is carried out in an aqueous environment. By using quartz crystal microbalance detection, short peptides can be monitored by their interaction with plastic antibodies specific for the target peptides. The selectivity of molecularly imprinted polymers and the sensitivity of such artificial biosensors have been combined to differentiate between traces of oxytocin and vasopressin to the ng mL(-1) scale.