Gobichelin A and B: Mixed-Ligand Siderophores Discovered Using Proteomics.
Gobichelin A and B: Mixed-Ligand Siderophores Discovered Using Proteomics.
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Gobichelin A 和 B：利用蛋白质组学发现的混合配体铁载体。
DOI：
10.1039/c2md20232h
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发表时间：
2013-01-01
期刊：
影响因子：
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通讯作者：
Kelleher NL
中科院分区：
文献类型：
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作者：
Chen Y;Unger M;Ntai I;McClure RA;Albright JC;Thomson RJ;Kelleher NL
“组学”策略已越来越多地应用于天然产物发现过程，迄今为止，许多实验室都实施了（元）基因组测序和挖掘。使用基于蛋白质组学的发现平台 PrISM（次级代谢蛋白质组学研究），我们从链霉菌属中发现了两种新的铁载体 gobichelin A 和 B。 NRRL F-4415，一种没有基因组测序的菌株。以蛋白质组学信息为指导，对负责戈比白蛋白生产的 37 kb 基因簇进行了测序，并将其 20 个开放阅读框解释为生物合成方案。这导致了对非核糖体肽（NRP）合成产生的新化合物的靶向检测和结构阐明。
“Omic” strategies have been increasingly applied to natural product discovery processes, with (meta-)genome sequencing and mining implemented in many laboratories to date. Using the proteomics-based discovery platform called PrISM (Proteomic Investigation of Secondary Metabolism), we discovered two new siderophores gobichelin A and B from Streptomyces sp. NRRL F-4415, a strain without a sequenced genome. Using the proteomics information as a guide, the 37 kb gene cluster responsible for production of gobichelins was sequenced and its 20 open reading frames interpreted into a biosynthetic scheme. This led to the targeted detection and structure elucidation of the new compounds produced by nonribosomal peptide (NRP) synthesis.