Functional Analysis of Gene-Silencing Suppressors from Tomato Yellow Leaf Curl Disease Viruses
Functional Analysis of Gene-Silencing Suppressors from Tomato Yellow Leaf Curl Disease Viruses
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DOI：
10.1094/mpmi-04-12-0094-r
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发表时间：
2012-10-01
影响因子：
3.5
通讯作者：
Bejarano, Eduardo R.
中科院分区：
文献类型：
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作者：
Luna, Ana P.;Morilla, Gabriel;Bejarano, Eduardo R.
番茄黄化曲叶病(TYLCD)是由番茄黄化卷叶病毒属(BegomVirus spp.)当它们感染番茄植株时会产生类似的症状，但寄主范围不同。在这项工作中，我们评估了从西班牙四种主要引起TYLCD的菌株中分离的C2、C4和V2病毒蛋白在本氏烟草中的基因沉默抑制活性。我们观察到每种被测试的病毒蛋白都有不同程度的局部沉默抑制，其中来自所有四种病毒的V2蛋白表现出最强的抑制活性。没有一个抑制者能够避免系统性沉默的蔓延，尽管大多数都产生了延迟。为了检测番茄黄化曲叶病毒(TYLCV)和番茄黄化曲叶病毒(TYLCSV)蛋白在共享寄主(番茄)和非共享寄主(豆类)中的沉默抑制活性，我们建立了新型斑点分析方法。利用这些工具，我们发现TYLCV的病毒蛋白能够抑制两种寄主的沉默，而TYLCSV蛋白只在番茄中有效。这是首次对来自致病双生病毒复合体的病毒抑制物进行全面分析，使用两种经济上重要的作物宿主，以及已建立的本氏新月蛾植物模型。
Tomato yellow leaf curl disease (TYLCD) is caused by a complex of phylogenetically related Begomovirus spp. that produce similar symptoms when they infect tomato plants but have different host ranges. In this work, we have evaluated the gene-silencing-suppression activity of C2, C4, and V2 viral proteins isolated from the four main TYLCD-causing strains in Spain in Nicotiana benthamiana. We observed varying degrees of local silencing suppression for each viral protein tested, with V2 proteins from all four viruses exhibiting the strongest suppression activity. None of the suppressors were able to avoid the spread of the systemic silencing, although most produced a delay. In order to test the silencing-suppression activity of Tomato yellow leaf curl virus (TYLCV) and Tomato yellow leaf curl Sardinia virus (TYLCSV) proteins in a shared (tomato) and nonshared (bean) host, we established novel patch assays. Using these tools, we found that viral proteins from TYLCV were able to suppress silencing in both hosts, whereas TYLCSV proteins were only effective in tomato. This is the first time that viral suppressors from a complex of disease-causing geminiviruses have been subject to a comprehensive analysis using two economically important crop hosts, as well as the established N. benthamiana plant model.