Isolation and characterization of the rye Waxy gene.
Isolation and characterization of the rye Waxy gene.
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DOI：
10.1139/g09-036
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发表时间：
2009-06
期刊：
影响因子：
3.1
通讯作者：
Jie Xu;M. Frick;A. Laroche;Z. Ni;Baojian Li;Zhen-Xiang Lu
中科院分区：
文献类型：
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作者：
Jie Xu;M. Frick;A. Laroche;Z. Ni;Baojian Li;Zhen-Xiang Lu
从黑麦基因组中分离出编码颗粒结合淀粉合成酶I（GBSSI）的WESTERN基因的完整基因组和cDNA序列，并进行了表征。结果表明，黑麦Wynth基因组DNA全长为2767 bp，cDNA全长为1815 bp。基因组序列有11个外显子，被10个内含子中断。黑麦Wessel基因富含GC，在编码区，尤其是密码子的第三位，GC频率较高。与其他谷类作物的同源序列相比，黑麦Wessel基因的外显子区域比内含子区域更保守。成熟黑麦GBSSI蛋白与小麦和大麦中的同源物具有超过95%的序列同一性。基于植物GBSSI蛋白序列比较的系统发育树显示了黑麦和其他植物基因组中WESIS基因之间的进化关系。黑麦WESTERN基因的鉴定将使黑麦和小黑麦中淀粉代谢的操纵成为可能。
Complete genomic and cDNA sequences of the Waxy gene encoding granule-bound starch synthase I (GBSSI) were isolated from the rye genome and characterized. The full-length rye Waxy genomic DNA and cDNA are 2767 bp and 1815 bp, respectively. The genomic sequence has 11 exons interrupted by 10 introns. The rye Waxy gene is GC-rich, with a higher GC frequency in the coding region, especially in the third position of the codons. Exon regions of the rye Waxy gene are more conserved than intron regions when compared with the homologous sequences of other cereals. The mature rye GBSSI proteins share more than 95% sequence identity with their homologs in wheat and barley. A phylogenetic tree based on sequence comparisons of available plant GBSSI proteins shows the evolutionary relationship among Waxy genes from rye and other plant genomes. The identification of the rye Waxy gene will enable the manipulation of starch metabolism in rye and triticale.