MATRICES CONTAINING GLYCOSAMINOGLYCANS IN THE DEVELOPING ANTERIOR CHAMBERS OF CHICK AND XENOPUS EMBRYONIC EYES
MATRICES CONTAINING GLYCOSAMINOGLYCANS IN THE DEVELOPING ANTERIOR CHAMBERS OF CHICK AND XENOPUS EMBRYONIC EYES
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DOI：
10.1016/0012-1606(79)90219-7
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发表时间：
1979-01-01
影响因子：
2.7
通讯作者：
ABBOTT, AS
中科院分区：
文献类型：
--
作者：
BARD, JBL;ABBOTT, AS
放射自显影和组织化学显示发育第 4 天鸡眼前房中存在的纤维基质含有硫酸软骨素、蛋白质、中性多糖和可能的透明质酸 (HA)。它的合成可能是由眼角的间充质完成的，大约需要 10 天的发育时间。扫描电子显微镜（SEM）显示，虽然基质随着眼睛的生长而变薄，但直到第15天才消失。描述了非洲爪蟾角膜的发育。从内角膜形成后不久（第 41 阶段）到 7 周后的变态，这种动物的前房中都有一个基质。在 SEM 中，这种材料表现为致密、无特征的聚集体，而不是粗纤维的基质；在透射电子显微镜下，它是一个含有暗色小颗粒的细丝网络。组织化学显示其含有HA、蛋白质和中性多糖。形态学证据与内角膜形成的基质相一致。基质的可能主要作用是在建立前房时将晶状体与角膜分离。在鸡胚中，基质也可能有助于稳定内皮细胞的形成过程。
The fibrous matrix present in the anterior chamber of the chick eye on the 4th day of development was shown by autoradiography and histochemistry to contain chondroitin sulfate, protein, neutral polysaccharides and possibly hyaluronic acid (HA). Its synthesis, probably by the mesenchyme of the angle of the eye, is completed by around 10 days of development. Scanning electron microscopy (SEM) shows that although the matrix thins as the eye grows, it does not disappear until the 15th day. The development of the Xenopus cornea is described. This animal has a matrix in its anterior chamber from soon after the formation of the inner cornea (stage 41) until metamorphosis 7 wk later. In the SEM, this material appears as a dense, featureless aggregate rather than as a matrix of thick fibers; in the transmission electron microscope, it is a network of fine filaments containing small dark-staining granules. Histochemistry shows that it contains HA, protein and neutral polysaccharides. The morphological evidence is compatible with the matrix being made by the inner cornea. The probable major role of the matrix is to separate lens from cornea in establishing the anterior chamber. In the chick embryo the matrix is also likely to help stabilize the endothelium during its formation.