Unique alignment and texture of biological apatite crystallites in typical calcified tissues analyzed by microbeam X-ray diffractometer system
Unique alignment and texture of biological apatite crystallites in typical calcified tissues analyzed by microbeam X-ray diffractometer system
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DOI：
10.1016/s8756-3282(02)00850-5
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发表时间：
2002-10-01
期刊：
影响因子：
4.1
通讯作者：
Umakoshi, Y
中科院分区：
文献类型：
--
作者：
Nakano, T;Kaibara, K;Umakoshi, Y
用微束X射线衍射仪研究了兔尺骨、兔颅骨和猴下颌骨典型钙化组织中生物磷灰石（Ap）晶粒的择优取向，以阐明Ap取向与力学功能之间的关系。通过（002）和（310）衍射峰之间的相对强度来评价生物Ap的c轴的优先排列。生物Ap在每个钙化组织中的优先排列根据体内的形状和应力条件而变化;也就是说，在兔尺骨和兔颅骨中的生物Ap的c轴分别优先观察为沿着纵轴的一维取向和沿着表面的二维取向。对沿着颅骨表面优先排列的精确分析显示，生物Ap的c轴呈椭圆形分布，沿着椎板外部和内部颅骨表面内的缝合线延伸。猴下颌骨生物学Ap的c轴在扁骨中基本上沿近远中方向沿着，但由于咀嚼力的作用，这种排列沿着平行于牙齿附近咬合方向的扁骨表面的法线方向发生变化。结果表明，微尺度测量生物Ap织构是评价钙化组织力学功能和应力分布的一种新方法。
Preferential orientation of biological apatite (Ap) crystallites in typical calcified tissues of rabbit ulna, rabbit skull, and monkey dentulous mandible was investigated using a microbeam X-ray diffractometer, with a beam spot of 100 mum in diameter, to clarify relationship between the Ap orientation and mechanical function. Preferential alignment of the c-axis of the biological Ap was evaluated by the relative intensity between (002) and (310) diffraction peaks. Preferential alignment of biological Ap in each calcified tissue varied depending on the shape and stress condition in vivo; that is, the c-axes of biological Ap in the rabbit ulna and the rabbit skull bone were preferentially observed as a one-dimensional orientation along the longitudinal axis and a two-dimensional orientation along the surface, respectively. Precise analysis of the preferential alignment along the skull surface showed an elliptical distribution of the c-axis of biological Ap elongating along the suture inside the skull surface of both lamina exterior and interior. The c-axis of biological Ap in a monkey dentulous mandible basically aligned along the mesiodistal direction in the flat bone, but this alignment changed along the normal direction to the flat bone surface parallel to the biting direction near the tooth, due to the force of mastication. It was concluded that the microscale measurement of biological Ap texture is one of the useful new methods for evaluating mechanical function and stress distribution in vivo in calcified tissues.