Possible association between amniotic fluid micro-organism infection and microflora in the mouth
Possible association between amniotic fluid micro-organism infection and microflora in the mouth
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DOI：
10.1016/s1470-0328(02)01349-6
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发表时间：
2002-05-01
影响因子：
5.8
通讯作者：
Allaker, RP
中科院分区：
文献类型：
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作者：
Bearfield, C;Davenport, ES;Allaker, RP
目的确定是否在羊膜腔中发现口腔细菌。设计实验室为基础的临床样本分析。设置皇家伦敦医院，白教堂。人口48名妇女参加择期剖腹产。方法牙菌斑，高阴道拭子，羊水和绒毛膜羊膜组织取自胎膜完整的妇女。主要结果测量使用培养和显微镜检查样本中是否存在微生物的通过聚合酶链反应（PCR）分析羊水中是否存在大多数真细菌特有的普遍存在的16 S rRNA基因。使用PCR属和种特异性引物分析样品，所述引物针对作为正常口腔微生物群落的一部分发现的细菌分类群（链球菌属（Streptococcus spp.）和具核梭杆菌（Fusobacterium nucleatum）。结果羊水中通用菌PCR、链球菌PCR及细胞因子PCR均为阳性。PCR和F.核质PCR阳性率分别为34/48、20/48和7/48。链球菌属和F.分别从48/48、14/48、0/48和29/48、6/48、0/48例受试者的牙菌斑、阴道和羊水中培养出有核细胞。发现微生物DNA（通用和F.与流产、宫内死亡、新生儿死亡、早产、胎膜早破等有显著性差异（P < 0.05和P < 0.01）。前列腺素E-2和细胞因子水平，与IL-1 α的例外，没有显着差异的妇女和没有证据的infection.Conclusions结果表明，链球菌属。和F.羊水中的核质可能有口腔起源。
Objective To determine whether oral bacteria are found in the amniotic cavity.Design Laboratory based analysis of clinical samples.Setting Royal London Hospital, Whitechapel.Population Forty-eight women attending for elective caesarean section.Methods Dental plaque, a high vaginal swab, amniotic fluid and chorioamnion tissue were taken from women with intact membranes.Main outcome measures Samples were investigated using culture and microscopy for the presence of microorganisms. Amniotic fluid was analysed by polymerase chain reaction (PCR) for the presence of the ubiquitous 16S rRNA gene specific to most eubacteria. Samples were analysed using PCR genus and species specific primers directed to bacterial taxa found as part of the normal oral microflora (Streptococcus spp. and Fusobacterium nucleatum). Levels of prostaglandin E-2 and cytokines were measured in amniotic fluid.Results Amniotic fluid was positive for universal bacteria PCR, Streptococcus spp. PCR and F. nucleatum PCR in 34/48, 20/48 and 7/48 of cases, respectively. Streptococcus spp. and F. nucleatum were cultured from the dental plaque, vagina and amniotic fluid of 48/48, 14/48, 0/48 and 29/48, 6/48, 0/48 subjects, respectively. A significant association was found between detection of microbial DNA (universal and F. nucletum) and complications in previous pregnancies including miscarriage, intrauterine death, neonatal death, preterm delivery and premature rupture of membranes (P < 0.05 and P < 0.01, respectively). Prostaglandin E-2 and cytokine levels, with the exception of IL-1alpha, were not significantly different between women with and without evidence of infection.Conclusions The results indicate that Streptococcus spp. and F. nucleatum in the amniotic fluid may have an oral origin.