STRUCTURE-FUNCTION-RELATIONSHIPS OF THE ADP/ATP CARRIER
STRUCTURE-FUNCTION-RELATIONSHIPS OF THE ADP/ATP CARRIER
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DOI：
10.1016/0005-2728(94)90119-8
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发表时间：
1994-08-30
影响因子：
4.3
通讯作者：
NELSON, DR
中科院分区：
文献类型：
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作者：
KLINGENBERG, M;NELSON, DR
线粒体含有大量溶质载体，负责线粒体内外空间之间的代谢物交换[1-3]。这些载体基于氨基酸序列形成基因家族。从ADP/ATP载体(AAC)[4]的一级序列开始，发现解偶联蛋白(UCP)[5]的序列是相似的。随后是磷酸盐载体的氨基酸序列，这坚定地证实了线粒体载体家族的存在[6]。随后，当酮戊二酸-苹果酸载体[7]和柠檬酸-苹果酸载体的结构变得已知时，这一点得到了扩展[8]。与此同时，在线粒体甚至其他细胞器中发现了一些具有与线粒体载体家族相似的结构动机的未知功能序列。因此，线粒体载体家族是“超家族”的一部分[9]。这些载体的特点是结构简单，仅包含约 300 个氨基酸[10]。可以从每个约100个残基的3倍相似结构域推断出内部3倍对称性。这些特征与任何其他已知的转运蛋白都没有相似之处。载体形成结构和功能上的同二聚体，这在载体中也是相当独特的。
Mitochondria contain a multitude of solute carriers which deal with the metabolite exchange between the inner-and extramitochondrial space [1-3]. These carriers form a gene family as based on the amino acid sequences. Starting with the primary sequence of the ADP/ATP carrier (AAC)[4], the sequence of the uncoupling protein (UCP)[5] was found to be similar. This was followed by the amino acid sequence of the phosphate carrier which firmly established the existence of a mitochondrial carrier family [6]. Subsequently, this was extended when the structures of the ketoglutarate-malate carrier [7] and the citrate-malate carrier became known [8]. In the meantime, several sequences of unknown function in mitochondria and even in other organelles have been found with a similar structural motive as the mitochondrial carrier family. For this reason the mitochondrial carrier family is part of a'superfamily'[9].These carriers are distinguished by the simplicity of their structures comprising only about 300 amino acids [10]. An internal 3-fold symmetry can be deduced from the 3-fold similar domains of about 100 residues each. These features have no similarity in any other transport protein known. The carriers form structurally and functionally homodimers which also is quite unique among carriers.