Heterogeneous expression of the cotton R2R3-MYB transcription factor GbMYB60 increases salt sensitivity in transgenic Arabidopsis
Heterogeneous expression of the cotton R2R3-MYB transcription factor GbMYB60 increases salt sensitivity in transgenic Arabidopsis
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棉花R2R3-MYB转录因子GbMYB60的异质表达增加转基因拟南芥的盐敏感性
DOI：
10.1007/s11240-017-1357-6
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发表时间：
2018-04-01
影响因子：
3
通讯作者：
Song, Chun-Peng
中科院分区：
文献类型：
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作者：
Xu, Fu-Chun;Liu, Hui-Li;Song, Chun-Peng
MYB蛋白是植物中最大的转录因子家族之一。越来越多的证据表明，MYB在植物生长发育的调节以及对非生物和生物胁迫的响应中起着重要作用。在本研究中，从海岛棉分离的GbMYB 60基因在拟南芥中异源表达。GbMYB 60编码具有转录激活活性的R2 R3-MYB蛋白。组织化学染色结果表明，GbMYB 60在幼苗的叶片维管和分生组织中以及成熟植株的腋芽组织中优先表达。表达模式分析表明GbMYB 60受阿坝、甘露醇和盐的诱导，而不受H2 O2的诱导。GbMYB 60基因在拟南芥中的高表达降低了拟南芥在盐和甘露醇处理下的发芽率，并降低了拟南芥成株期的耐盐性。转基因拟南芥积累更多的活性氧相比，野生型植物在胁迫条件下，相应的转基因植物中更严重的细胞死亡。此外，ABA相关基因，包括ABI 5和ABF 3，在GbMYB 60过表达的植物中下调。
MYB proteins form one of the largest transcription factor families in plants. Growing evidence has suggested that MYB has important roles in the regulation of plant growth and development, as well as responses to abiotic and biotic stresses. In the present study, the GbMYB60 gene isolated from Gossypium barbadense was heterogeneously expressed in Arabidopsis. GbMYB60 encodes a R2R3-MYB protein with transcriptional activation activity. Histochemical staining revealed that GbMYB60 was preferential expressed in the leaf vascular and meristem tissues of young seedlings and in the axillary bud tissue of mature plants. Expression pattern analysis indicated that GbMYB60 is induced by ABA, mannitol, and salt, but not H2O2. Transgenic Arabidopsis with constitutively higher expression of GbMYB60 exhibited reduced germination rates under salt and mannitol treatments as well as reduced tolerance to salt at the adult-plant stage. Transgenic Arabidopsis accumulates more reactive oxygen species compared to wild-type plants under stress conditions, corresponding with more severe cell death in transgenic plants. Additionally, ABA-related genes, including ABI5 and ABF3, were down-regulated in GbMYB60-overexpressing plants.