Mechanistic insights into toxicity pathways induced by nanomaterials in Daphnia magna from analysis of the composition of the acquired protein corona
Mechanistic insights into toxicity pathways induced by nanomaterials in Daphnia magna from analysis of the composition of the acquired protein corona
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DOI：
10.1039/d0en00625d
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发表时间：
2020-11-01
影响因子：
7.3
通讯作者：
Lynch, Iseult
中科院分区：
文献类型：
--
作者：
Ellis, Laura-Jayne A.;Lynch, Iseult
鉴定存在于血浆、血清和细胞裂解物培养的纳米材料（NMs）表面的蛋白质，可以深入了解纳米材料的生物相互作用，包括摄取和毒性。水蚤（Daphnia magna）等生物通过分泌蛋白质和其他生物分子对有毒物质做出反应；我们证明，在暴露于NMs期间获得的生态电晕可以提供类似的重要见解，关于NMs暴露诱导的机制途径，作为生态毒性评估的一部分。采用新鲜分散的（原始的）和“中等老化的”纳米颗粒（作为环境污染物），不同的核心材料、大小和表面性质，研究了纳米颗粒物理化学特性和暴露环境对模型试验物种水蚤体内蛋白质电晕组成和吸收潜力的影响。表面结合蛋白电晕的组成随着纳米颗粒类型、年龄和培养环境的不同而改变，从而改变了纳米颗粒的生物学特性和相互作用。在新分散的纳米表面上发现的蛋白质主要与代谢损伤、DNA损伤、线粒体分解和能量过程有关，所有这些都与细胞毒性损伤有关。与新鲜分散的NMs相比，在所有培养基条件下，与老化NMs结合的蛋白质明显减少。仅与老年NMs结合的蛋白质参与钙离子结合和细胞氧化还原稳态，表明对老年NMs的毒性反应明显较低。因此，纳米蛋白冠组成可以促进检测生物体对纳米暴露的反应，并可能识别不良结果途径中的分子启动事件。
Identifying proteins present on the surface of nanomaterials (NMs) incubated with plasma, serum, and cell lysates can offer insights into the biological interactions of the NMs, including uptake and toxicity. Organisms such as Daphnia magna respond to the presence of toxicants by secreting proteins and other biomolecules; we demonstrate that the eco-corona acquired during exposure to NMs can provide similar important insights regarding the mechanistic pathways induced by the NM exposure as part of an ecotoxicity assessment. Using freshly dispersed (pristine) and 'medium aged' NMs (as environmental pollutants), differing in core material, size and surface properties, the influence of NM physicochemical characteristics and exposure environment on the protein corona composition and potential for in vivo uptake by the model test species Daphnia magna are investigated. Surface bound protein corona compositions changed between NM type, NM 'age' and the environmental medium in which the NMs were incubated, thus changing the NM biological identity and interactions. Proteins identified on the freshly dispersed NM surfaces were largely associated with metabolic damage, DNA damage, mitochondrial breakdown and energy processes, all of which are associated with cytotoxic damage. Significantly fewer proteins were bound to the aged NMs in all medium conditions, compared to the freshly dispersed NMs. Proteins bound only to the aged NMs were involved in calcium ion binding and cell redox homeostasis, indicative of significantly lower toxic responses to the aged NMs. Thus, NM protein corona composition can facilitate detection of organism responses to NM exposure and potentially identification of the molecular initiating events in adverse outcome pathways.