Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization.
Physical clustering of FLC alleles during Polycomb-mediated epigenetic silencing in vernalization.
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DOI：
10.1101/gad.221713.113
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发表时间：
2013-09-01
影响因子：
10.5
通讯作者：
Dean C
中科院分区：
文献类型：
--
作者：
Rosa S;De Lucia F;Mylne JS;Zhu D;Ohmido N;Pendle A;Kato N;Shaw P;Dean C
核组织如何影响单个位点的染色质变化还知之甚少。春化作用，通过寒冷促进开花，涉及Polycomb介导的FLC基因座C沉默。在这里，Rosa等人使用活细胞成像来监测春化过程中的核组织和FLC动态。这些数据表明，FLC等位基因的Polycomb依赖性聚类是基因沉默中的冷诱导步骤。这项研究表明，靶基因的物理聚类可能是Polycomb介导的表观遗传沉默机制的共同特征。春化作用，通过寒冷促进开花，涉及Polycomb介导的FLC基因座C的表观遗传沉默。冷逐渐促进细胞自主切换到沉默状态。在这里，我们使用FLC-lacO的活细胞成像来监测春化过程中核组织的变化。FLC-lacO等位基因在寒冷期间物理聚集，并且在植物恢复温暖后通常保持如此。成簇依赖于建立FLC沉默状态所必需的多梳型反式因子，而不依赖于维持沉默功能的异染色质样蛋白1。这些数据支持物理聚类可能是Polycomb介导的表观遗传转换机制的共同特征的观点。
How nuclear organization influences chromatin changes at individual loci is poorly understood. Vernalization, the promotion of flowering by cold, involves Polycomb-mediated silencing of FLOWERING LOCUS C (FLC). Here, Rosa et al. use live-cell imaging to monitor nuclear organization and FLC dynamics during vernalization. The data reveal that Polycomb-dependent clustering of FLC alleles is a cold-induced step in gene silencing. This study suggests that physical clustering of target genes may be a common feature of Polycomb-mediated epigenetic silencing mechanisms. Vernalization, the promotion of flowering by cold, involves Polycomb-mediated epigenetic silencing of FLOWERING LOCUS C (FLC). Cold progressively promotes cell-autonomous switching to a silenced state. Here, we used live-cell imaging of FLC-lacO to monitor changes in nuclear organization during vernalization. FLC-lacO alleles physically cluster during the cold and generally remain so after plants are returned to warm. Clustering is dependent on the Polycomb trans-factors necessary for establishment of the FLC silenced state but not on LIKE HETEROCHROMATIN PROTEIN 1, which functions to maintain silencing. These data support the view that physical clustering may be a common feature of Polycomb-mediated epigenetic switching mechanisms.
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