The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF-κB pathway in non-small cell lung cancer cell lines A549 and NCI-H1299
The antitumor effect of hinesol, extract from Atractylodes lancea (Thunb.) DC. by proliferation, inhibition, and apoptosis induction via MEK/ERK and NF-κB pathway in non-small cell lung cancer cell lines A549 and NCI-H1299
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苍术提取物 Hinesol 的抗肿瘤作用。
DOI：
10.1002/jcb.28696
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发表时间：
2019-07-24
影响因子：
4
通讯作者：
Li, Liangzhi
中科院分区：
文献类型：
--
作者：
Guo, Weigiang;Liu, Songbai;Li, Liangzhi
肺癌（尤其是非小细胞肺癌[NSCLC]）是世界上最恶性的癌症之一。白藜芦醇是茅苍术挥发油的主要成分。DC是最有前途的抗肿瘤细胞。然而，其抗非小细胞肺癌细胞增殖的作用及其分子机制尚不清楚。在本研究中，我们发现hinesol有效地抑制A549和NCI-H1299细胞以剂量和时间依赖的方式通过3-（4，5-二甲基噻唑-2-基）-2，5-二苯基四唑溴化物测定。流式细胞仪检测结果显示，hinesol可使A549细胞周期阻滞于G 0/G1期，并诱导A549细胞凋亡。Western blot结果显示，hinesol可降低丝裂原活化蛋白激酶（MAPK）、细胞外信号调节激酶（ERK）、I κ B α的磷酸化水平，抑制Bcl-2、cyclin D1的表达，上调Bax的表达。因此，hinesol有可能成为一种潜在的抗非小细胞肺癌候选药物。
Lung cancer (especially, non-small cell lung cancer [NSCLC]) is one of the most malignant cancers in the world. Hinesol is the major component of the essential oil of Atractylodes lancea (Thunb.) DC and possesses the most promising anticancer function. However, the effects and molecular mechanism of hinesol on antiproliferation in NSCLC cells has not been well understood. In this study, we found that hinesol effectively inhibited the A549 and NCI-H1299 cells in a dose- and time-dependent manner by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide assay. In addition, hinesol induced cell cycle arrest at G0/G1 phase and apoptosis assessed by flow cytometry in A549 cells. Furthermore, Western blot analysis showed that hinesol decreased phosphorylation of mitogen-activated protein kinase, extracellular signal-regulated kinase, I kappa B alpha, and p65 inhibited the expressions of Bcl-2, cyclin D1 and upregulated the expression of Bax. Based on these results, hinesol might be a potential drug candidate of anti-NSCLC for therapy.