HUMORAL IMMUNE-RESPONSE TO OUTER SURFACE PROTEIN-C OF BORRELIA-BURGDORFERI IN LYME-DISEASE - ROLE OF THE IMMUNOGLOBULIN-M RESPONSE IN THE SERODIAGNOSIS OF EARLY INFECTION
HUMORAL IMMUNE-RESPONSE TO OUTER SURFACE PROTEIN-C OF BORRELIA-BURGDORFERI IN LYME-DISEASE - ROLE OF THE IMMUNOGLOBULIN-M RESPONSE IN THE SERODIAGNOSIS OF EARLY INFECTION
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DOI：
10.1128/iai.62.8.3213-3221.1994
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发表时间：
1994-08-01
影响因子：
3.1
通讯作者：
STEERE, AC
中科院分区：
文献类型：
--
作者：
FUNG, BP;MCHUGH, GL;STEERE, AC
我们测定了莱姆病早期或晚期症状患者对伯氏疏螺旋体外表面蛋白 C (OspC) 的体液免疫反应，并研究了该抗原在早期感染血清诊断中的用途。来自低传代人分离株 297（北美伯氏疏螺旋体菌株）的 ospC 基因用于制备重组麦芽糖结合蛋白 (MBP)-OspC 融合蛋白，用于血清学测试。该基因与伯氏疏螺旋体 B31 和 2591 的 ospC 具有 84% 至 85% 的核苷酸序列同一性和 76% 至 79% 的氨基酸同一性。对 MBP-OspC 的抗体反应是在 15 名莱姆病患者的连续血清中测定的，这些患者被监测了 4 至 12 年的疾病，在来自 189 名患有该疾病的早期或晚期表现的患者的单血清样本中，以及在来自106 名对照患者。在感染早期，患有游走性红斑或脑膜炎的患者通常会对 OspC 产生弱到强的免疫球蛋白 M (IgM) 反应，有时对 IgG 反应弱到中度。几个月到几年后，在关节炎患者中，这种蛋白质的 IgG 反应通常很明显，从弱到强，但在慢性神经疏螺旋体病患者中，这种反应很弱或不存在。当对游走性红斑患者的急性期和恢复期血清样本进行 MBP-OspC 反应性测试时，无论采用酶联免疫吸附测定 (ELISA) 或蛋白质印迹法，IgM 测试的敏感性为 73%，特异性为 98%。我们得出的结论是，大多数莱姆病患者在疾病早期对 OspC 具有显着的 IgM 反应，随后关节炎患者通常会出现显着的 IgG 反应。对于早期感染的血清诊断，当使用MBP-OspC作为抗原时，与使用螺旋体裂解物的测试相比，IgM ELISA和Western blotting的敏感性和特异性相当或略有提高。
We determined the humoral immune response to outer surface protein C (OspC) of Borrelia burgdorferi in patients with early or late manifestations of Lyme disease and investigated the use of this antigen in the serodiagnosis of early infection. The ospC gene from the low-passage human isolate 297, a North American B. burgdorferi strain, was used to make a recombinant maltose-binding protein (MBP)-OspC fusion protein for serologic tests. This gene showed 84 to 85% nucleotide sequence identity and 76 to 79% amino acid identity with ospC of B. burgdorferi B31 and 2591. The antibody responses to MBP-OspC were determined in serial sera from 15 patients with Lyme disease who were monitored for 4 to 12 years of illness, in single-serum samples from 189 patients with early or late manifestations of the disorder, and in serum samples from 106 control patients. Early in the infection, patients with erythema migrans or meningitis commonly had weak to strong immunoglobulin M (IgM) responses to OspC and sometimes weak to moderate IgG responses. Months to years later, weak to strong IgG reactivity with this protein was often apparent in patients with arthritis, but this response was weak or absent in patients with chronic neuroborreliosis. When acute and convalescent-phase serum samples from patients with erythema migrans were tested for reactivity against MBP-OspC, the sensitivity of the IgM test was 73% and the specificity was 98%, with either enzyme-linked immunosorbent assay (ELISA) or Western blotting. We conclude that the majority of patients with Lyme disease have a prominent IgM response to OspC early in the illness, which is often followed by a prominent IgG response in patients with arthritis. For the serodiagnosis of early infection, the sensitivity and specificity of IgM ELISA and Western blotting were comparable or slightly improved when MBP-OspC was used as the antigen compared with tests in which spirochetal lysates were used.