Effects of intermittent hyperbaric exposure on endurance and interval exercise performance in well‐trained mice
Effects of intermittent hyperbaric exposure on endurance and interval exercise performance in well‐trained mice
复制标题
间歇性高压暴露对训练有素的小鼠耐力和间歇运动表现的影响
DOI：
10.1113/ep087360
复制
发表时间：
2019
影响因子：
2.7
通讯作者：
Suzuki Junichi
中科院分区：
文献类型：
--
作者：
志賀充;Junichi SUZUKI;TAKANORI ISHII;志賀充;石井隆憲;Suzuki Junichi
新发现这项研究的中心问题是什么？间歇性高压暴露(1.3个大气压，含20.9%O2)通过促进骨骼肌的氧化和糖酵解能力来提高耐力。目前尚不清楚这一策略是否提高了训练有素的个体的耐力表现。主要发现及其重要性是什么？高压氧暴露结合耐力训练可以增强氧化和糖酵解能力，以及线粒体转录因子A、动力蛋白相关蛋白-1和热休克蛋白70的蛋白水平。高压氧暴露加短跑间歇训练增加了I型肌纤维的比例，促进了毛细血管生长和肌纤维肥大。本研究旨在阐明高压氧暴露(1.3个大气压，含20.9%O2)提高高训练小鼠耐力和间歇运动能力的机制。训练组雄性小鼠从5周龄开始被关在带轮子活动装置的笼子里7周。自愿跑步显著提高了最大耐力6.4倍。训练后的小鼠分别接受耐力跑步机训练(20~32.5m/min−1)或短跑间歇训练(S跑~10min S休息30~42.5m/min−1)和不进行1h高压氧暴露(分别为ET或SIT)，共4周。HypET和HypSIT显著提高最大耐力，HypSIT显著提高最大间歇耐力。HypET和HypSIT后，过氧化体增殖物激活受体-γ共激活因子1-α的表达水平显著升高。HypET使红色腓肠肌的3-羟基酰辅酶A脱氢酶、柠檬酸合成酶和磷酸果糖激酶的活性水平明显高于ET。HypET组线粒体转录因子A、动力素相关蛋白-1和热休克蛋白-70的蛋白水平高于ET组。HypSIT能显著增加比目鱼肌中I型纤维的比例。HypSIT较SIT对白色腓肠肌毛细血管纤维比值的增加更明显。这些结果表明，高压氧暴露对高训练小鼠的耐力和间歇训练有好处，以提高运动能力。
New FindingsWhat is the central question of this study?Intermittent hyperbaric exposure (1.3 atmospheres absolute with 20.9% O2) enhances endurance capacity by facilitating oxidative and glycolytic capacities in skeletal muscle. It remains unclear whether this strategy enhances endurance performance in well‐trained individuals.What is the main finding and its importance?Hyperbaric exposure with endurance training enhanced oxidative and glycolytic capacities and protein levels of mitochondrial transcription factor A, dynamin‐related protein‐1 and heat shock protein 70. Hyperbaric exposure with sprint interval training increased the proportion of type I muscle fibres and promoted capillary growth and muscle fibre hypertrophy. These results may lead to a new strategy for enhancing exercise capacity in well‐trained mice.AbstractThe study was designed to clarify the mechanisms by which hyperbaric exposure (1.3 atmospheres absolute with 20.9% O2) improves endurance and interval exercise capacities in highly trained mice. Male mice in the training group were housed in a cage with a wheel activity device for 7 weeks from 5 weeks old. Voluntary running markedly increased maximal endurance capacity by 6.4‐fold. Trained mice were then subjected to either endurance treadmill training (20–32.5 m min−1) or sprint interval training (5 s run–10 s rest, 30–42.5 m min−1) with (HypET or HypSIT, respectively) and without (ET or SIT, respectively) 1 h hyperbaric exposure for 4 weeks. Maximal endurance capacity was significantly increased by HypET and HypSIT, and maximal interval capacity was significantly enhanced by HypSIT. Peroxisome proliferator‐activated receptor gamma coactivator 1‐alpha expression levels were markedly increased after HypET and HypSIT. Activity levels of 3‐hydroxyacyl‐CoA‐dehydrogenase, citrate synthase and phosphofructokinase in the red gastrocnemius muscle were increased more by HypET than by ET. Protein levels of mitochondrial transcription factor A, dynamin‐related protein‐1 and heat shock protein 70 were increased more by HypET than by ET. The proportion of type I fibres in the soleus muscle was remarkably increased by HypSIT. Capillary‐to‐fibre ratio values in the white gastrocnemius were increased more by HypSIT than by SIT. These results suggest that hyperbaric exposure has beneficial effects for endurance and interval training to improve exercise capacity in highly trained mice.