High genomic diversity of enterohemorrhagic Escherichia coli isolates in Japan and its applicability for the detection of diffuse outbreak.
High genomic diversity of enterohemorrhagic Escherichia coli isolates in Japan and its applicability for the detection of diffuse outbreak.
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日本肠出血性大肠杆菌分离株的高基因组多样性及其在检测弥漫性疫情中的适用性。
DOI：
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发表时间：
2002
影响因子：
2.2
通讯作者：
Haruo Watanabe
中科院分区：
文献类型：
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作者：
J. Terajima;H. Izumiya;S. Iyoda;K. Tamura;Haruo Watanabe
2000年1月至11月，通过使用脉冲场凝胶电泳（PFGE）对1，102株肠出血性大肠杆菌分离株进行基因分型，发现日本这些分离株的基因组多样性很高。通过将分离株的基因分型结果与其他病例流行病学调查的信息相结合，我们确定了2000年日本的一次弥漫性疫情，该疫情似乎是散发的，但实际上是相互关联的。从患者标本和这次扩散性疫情中涉及的污染食品中分离出的仅具有滋贺毒素2基因的菌株显示出难以区分的PFGE谱和相同的噬菌体类型。根据肠出血性E.大肠杆菌O 157：H7/-在日本，我们建议存在一些其他可能的扩散爆发，由于生物体，显示出难以区分的基因型。
Genotyping of 1,102 enterohemorrhagic Escherichia coli isolates by the use of pulsed-field gel electrophoresis (PFGE) carried out from January to November 2000 has revealed the high genomic diversity of these isolates in Japan. By combining the results of genotyping of the isolates with the information from other epidemiological investigations of the cases, we identified a diffuse outbreak in Japan in the year 2000 that seemed to be sporadic but was actually linked. Isolates with only the Shiga toxin 2 gene derived from patient specimens and the contaminated food involved in this diffuse outbreak showed an indistinguishable PFGE profile and the same phage type. Based on the diversity of genotypes among the isolates of enterohemorrhagic E. coli O157:H7/- in Japan, we suggest the presence of a few other possible diffuse outbreaks due to the organisms, showing indistinguishable genotypes.