Cartilage mechanical response under dynamic compression at physiological stress levels following collagenase digestion
Cartilage mechanical response under dynamic compression at physiological stress levels following collagenase digestion
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DOI：
10.1007/s10439-007-9431-6
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发表时间：
2008-03-01
影响因子：
3.8
通讯作者：
Ateshian, Gerard A.
中科院分区：
文献类型：
--
作者：
Park, Seonghun;Nicoll, Steven B.;Ateshian, Gerard A.
本研究的目的是测试的假设，胶原酶的酶降解显着降低动态模量和增加生理压缩应力水平和加载频率下的牛关节软骨的压缩应变。在含有不同浓度胶原酶（0、2和10 U/mL）的生理缓冲盐水中孵育之前和之后，将27个股骨远端软骨栓（直径3 mm）在负荷控制下加载到定制装置中，负荷高达40 N，加载频率为0.1、1、10和40 Hz。相对于相应对照，胶原酶消化使平衡杨氏模量在2U/mL下降低49%，在10 U/mL下降低61%，而在40 Hz下动态模量在2U/mL下降低13-20%，在10 U/mL下降低24-33%。使用10 U/mL胶原酶，动态压缩应变的幅度在0.1 Hz时从22 +/- 6%增加到26 +/- 8%，在40 Hz时从9.6 +/- 3.3%增加到13.5 +/- 3.2%。该实验研究用于证实胶原蛋白对软骨的动态压缩特性有显著贡献，通过证明胶原酶消化在代表生理负荷条件的应力幅度和频率下损害这些特性。
The objective of this study was to test the hypothesis that enzymatic degradation by collagenase significantly reduces dynamic moduli and increases compressive strains of bovine articular cartilage under physiological compressive stress levels and loading frequencies. Twenty-seven distal femoral cartilage plugs (3 mm diameter) were loaded in a custom apparatus under load control, with a load up to 40 N and loading frequencies of 0.1, 1, 10, and 40 Hz, before and after incubation in physiological buffered saline containing various concentrations of collagenase (0, 2, and 10 U/mL). Collagenase digestion reduced the equilibrium Young's modulus by 49% with 2 U/mL and 61% with 10 U/mL, while the decrease in dynamic modulus at 40 Hz was in the range of 13-20% with 2 U/mL and 24-33% with 10 U/mL, relative to respective controls. The amplitudes of dynamic compressive strains increased from 22 +/- 6% to 26 +/- 8% at 0.1 Hz and 9.6 +/- 3.3% to 13.5 +/- 3.2% at 40 Hz, with 10 U/mL collagenase. This experimental study serves to confirm that collagen contributes significantly to the dynamic compressive properties of cartilage, by demonstrating that collagenase digestion impairs these properties, under stress amplitudes and frequencies which are representative of physiological loading conditions.