Estimating weak ratiometric signals in imaging data. II. Meta-analysis with multiple, dual-channel datasets.
Estimating weak ratiometric signals in imaging data. II. Meta-analysis with multiple, dual-channel datasets.
复制标题
DOI：
10.1364/josaa.25.002185
复制
发表时间：
2008-09
期刊：
影响因子：
--
通讯作者：
A. Sornborger;J. Broder;A. Majumder;Ganesh Srinivasamoorthy;E. Porter;Sean S Reagin;Charles Keith;J. Lauderdale
中科院分区：
文献类型：
--
作者：
A. Sornborger;J. Broder;A. Majumder;Ganesh Srinivasamoorthy;E. Porter;Sean S Reagin;Charles Keith;J. Lauderdale
比率荧光指示剂用于对各种生理变量进行定量测量。它们的效用往往受到噪音的限制。这是一系列论文中的第二篇，描述了用于对比率计量数据进行去噪的统计方法，目的是获得感兴趣的变量的改进的定量估计。在这里，我们概述了一个统计优化方法，设计用于分析的比率成像数据，其中多个测量已采取的系统响应相同的刺激协议。该方法利用多个数据集之间的相关信息来客观地检测和估计比率信号。我们证明了我们的方法，显示其应用程序的多个，比率钙成像实验的结果。
Ratiometric fluorescent indicators are used for making quantitative measurements of a variety of physiological variables. Their utility is often limited by noise. This is the second in a series of papers describing statistical methods for denoising ratiometric data with the aim of obtaining improved quantitative estimates of variables of interest. Here, we outline a statistical optimization method that is designed for the analysis of ratiometric imaging data in which multiple measurements have been taken of systems responding to the same stimulation protocol. This method takes advantage of correlated information across multiple datasets for objectively detecting and estimating ratiometric signals. We demonstrate our method by showing results of its application on multiple, ratiometric calcium imaging experiments.