Ectomycorrhizal fungal community structure across a bog-forest ecotone in southeastern Alaska
Ectomycorrhizal fungal community structure across a bog-forest ecotone in southeastern Alaska
复制标题
阿拉斯加东南部沼泽森林交错带的外生菌根真菌群落结构
作者：
N. Wurzburger;A. Hartshorn;R. Hendrick
我们研究了阿拉斯加东南部沼泽森林交错带的外生菌根(ECM)真菌群落。沼泽和边缘的特征都是排水不良的组织土壤和连续的一层泥炭属植物、灌木、苔草属植物和海岸松树[Pinus trorta Dougl.前大声。瓦尔访问数/每百万人：Reach for[Contorta].森林中有排水较好的Inceptisols和Spodosol，这是一个由西部铁杉组成的乔木群落[Tsuga Heterophylla(Raf.)、黄柏(Thuja plicata donn ex D.Don.)、锡特卡云杉[Picea Sitchens(Bong.)Carr.]和海岸松，以及灌木和草本植物的灌木丛。森林中的根尖密度(TIPS cm，−，3土)显着大于边缘或沼泽，3种植物群落中的根尖密度也存在显着差异。利用分子生物学技术(聚合酶链式反应-限制性片段长度多态性和DNA测序)对地下部分ECM真菌类群进行分析。3个ECM真菌分类群：毛状链霉菌(Suillustomentosus(Kauffman)Singer)、地球嗜地空心菌(Cenodiccum Geophilum Fr.：FR)和红曲霉属(Russula)的相对频率不同，但在所有3个植物群落中属于最常见的4个。虽然不同植物群落的ECM真菌丰富度存在差异，但由于根密度和定殖率的差异，ECM根部取样不均匀，混淆了丰富度比较。使用重采样程序来创建分类单元积累曲线作为采样的ECM根的函数，揭示了整个交错带中累积的ECM真菌分类群丰富度的相似性。
We examined the ectomycorrhizal (ECM) fungal community across a bog-forest ecotone in southeastern Alaska. The bog and edge were both characterized by poorly drained Histosols and a continuous layer of Sphagnum species, ericaceous shrubs, Carex species, and shore pine [Pinus contorta Dougl. ex Loud. var. contorta]. The forest had better-drained Inceptisols and Spodosols, a tree community comprised of western hemlock [Tsuga heterophylla (Raf.) Sarg.], yellow cedar (Thuja plicata Donn ex D. Don.), Sitka spruce [Picea sitchensis (Bong.) Carr.] and shore pine, and an understorey of ericaceous shrubs and herbs. ECM root tip density (tips cm−3 soil) was significantly greater in the forest than the edge or bog and ECM colonization was significantly different in all three plant communities. The below ground ECM fungal taxa were analyzed using molecular techniques (PCR-RFLP and DNA sequencing). Three ECM fungal taxa, Suillus tomentosus (Kauffman) Singer, Cenococcum geophilum Fr.:Fr, and a Russula species, differed in relative frequency, yet were among the four most frequent in all three plant communities. Although differences in ECM fungal richness were observed across plant communities, unequal sampling of ECM roots due to root density and colonization differences confounded richness comparisons. Using resampling procedures for creating taxon-accumulation curves as a function of sampled ECM roots revealed similarities in cumulative ECM fungal taxa richness across the ecotone.