Drosophila caspase DRONC is required for specific developmental cell death pathways and stress-induced apoptosis
Drosophila caspase DRONC is required for specific developmental cell death pathways and stress-induced apoptosis
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DOI：
10.1016/j.devcel.2004.09.018
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发表时间：
2004-12-01
影响因子：
11.8
通讯作者：
Kumar, S
中科院分区：
文献类型：
--
作者：
Daish, TJ;Mills, K;Kumar, S
Caspase 家族的蛋白酶在细胞凋亡的执行中发挥关键作用。果蝇中有七种半胱天冬酶，但由于缺乏特定的突变体，它们在细胞死亡中的作用尚未得到详细研究。在这里，我们描述了编码 DRONC 的果蝇基因的特定突变体的产生，DRONC 是果蝇中唯一含有顶端 caspase 的 caspase 募集结构域 (CARD)。 dronc 突变体是蛹致死的，我们的研究表明 DRONC 是 DNA 损伤诱导的许多形式的发育细胞死亡和细胞凋亡所必需的。此外，我们证明 DRONC 是幼虫唾液腺在变态过程中自噬死亡所必需的，但不是幼虫中肠的组织溶解所必需的。我们的结果表明，DRONC 参与特定的发育细胞死亡途径，并且在某些组织中，效应器 caspase 激活和细胞死亡可以独立于 DRONC 发生。
Proteases of the caspase family play key roles in the execution of apoptosis. In Drosophila there are seven caspases, but their roles in cell death have not been studied in detail due to a lack of availability of specific mutants. Here, we describe the generation of a specific mutant of the Drosophila gene encoding DRONC, the only caspase recruitment domain (CARD) containing apical caspase in the fly. dronc mutants are pupal lethal and our studies show that DRONC is required for many forms of developmental cell deaths and apoptosis induced by DNA damage. Furthermore, we demonstrate that DRONC is required for the autophagic death of larval salivary glands during metamorphosis, but not for histolysis of larval midguts. Our results indicate that DRONC is involved in specific developmental cell death pathways and that in some tissues, effector caspase activation and cell death can occur independently of DRONC.