The complete mitochondrial genome of Corydoras nattereri (Callichthyidae: Corydoradinae)

The complete mitochondrial genome of Corydoras nattereri (Callichthyidae: Corydoradinae)
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DOI:
10.1590/1982-0224-20150167
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发表时间:
2016-01-01
影响因子:
1.7
通讯作者:
Parente, Thiago E.
Parente, Thiago E.
中科院分区:
生物学4区
文献类型:
--
作者:
Moreira, Daniel A.;Buckup, Paulo A.;Parente, Thiago E.

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利用新一代测序技术,对巴西东南部的一种银鲶(Corydoras nattereri)进行了完整的有丝分裂基因组重建。利用来自3个个体肝脏转录组的线粒体转录本组装有丝分裂基因组,并产生16,557个核苷酸的环状DNA序列,编码22个tRNA基因,2个rRNA基因,13个蛋白质编码基因和2个非编码控制区(D-loop, OrigL)。细胞色素氧化酶C亚基I (COI)近缘序列的系统地理分析表明,在形态相似的柽柳种群中存在高度多样性。蝶蛹是在一个复杂的种群中筑巢的,目前被分配给古蝶和艾哈蒂。有丝分裂基因组结构分析表明,在ATPase亚基-6和COIII基因之间插入21个核苷酸可能代表了与紫堇科进化相关的系统发育信息特征。
The complete mitogenome of Corydoras nattereri, a species of mailed catfishes from southeastern Brazil, was reconstructed using next-generation sequencing techniques. The mitogenome was assembled using mitochondrial transcripts from the liver transcriptomes of three individuals, and produced a circular DNA sequence of 16,557 nucleotides encoding 22 tRNA genes, two rRNA genes, 13 protein-coding genes and two noncoding control regions (D-loop, OrigL). Phylogeographic analysis of closely related sequences of Cytochrome Oxydase C subunit I (COI) demonstrates high diversity among morphologically similar populations of C. nattereri. Corydoras nattereri is nested within a complex of populations currently assigned to C. paleatus and C. ehrhardti. Analysis of mitogenome structure demonstrated that an insertion of 21 nucleotides between the ATPase subunit-6 and COIII genes may represent a phylogenetically informative character associated with the evolution of the Corydoradinae.