The subpopulation of mesenchymal stem cells that differentiate toward cardiomyocytes is cardiac progenitor cells
The subpopulation of mesenchymal stem cells that differentiate toward cardiomyocytes is cardiac progenitor cells
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分化为心肌细胞的间充质干细胞亚群是心脏祖细胞。
DOI：
10.1016/j.yexcr.2011.08.011
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发表时间：
2011-11-01
影响因子：
3.7
通讯作者：
Ma, Aiqun
中科院分区：
文献类型：
--
作者：
Wei, Feng;Wang, Tingzhong;Ma, Aiqun
间充质干细胞（MSCs）被认为是一种有前途的心脏损伤的细胞治疗来源。完整的，少于30%的MSC有助于心肌细胞分化，并且该细胞群体的分离程序和生物学特性仍然未知。在这里，我们分离和调查的生物学特性的MSC的这个亚群。使用单细胞单克隆技术随机选择24个MSC克隆。经5-氮胞苷诱导后，8个克隆呈现心肌细胞样形态，心肌特异性标志物cTnT和α-actin高表达（90%以上），并显示心肌细胞典型的瞬时外向钾电流（I-to）、内向整流钾电流（I-K1）和延迟整流钾电流（I-KDR）。其他克隆仅显示I-to电流，且电流密度与心肌细胞不同。与其他克隆相比，在用5-氮杂胞苷诱导之前，8个克隆表达早期心脏标志物GATA 4和NKX2.5，但不表达cTnT、α-肌动蛋白、CD 44和CD 90，并且在诱导后不具有脂肪生成、骨生成或软骨生成的潜力。我们的数据表明，有助于心肌细胞分化的MSC亚群是心脏祖细胞。此外，我们使用单细胞单克隆技术初步纯化了具有高心肌细胞分化潜力的细胞群。(C)2011 Elsevier Inc. All rights reserved.
Mesenchymal stem cells (MSCs) are regarded as a promising source of cell-based therapy for heart injury. Intact, less than 30% of MSCs contribute to cardiomyocytes differentiation, and the isolation procedure and biological characteristics of this population of cells remain unknown. Here we isolate and investigate the biological characteristics of this subpopulation of MSCs. Twenty four MSC clones were randomly selected using single-cell monoclonal technology. After induced with 5-azacytidine, eight clones displayed cardiomyocyte-like morphologies, and highly (over 90%) expressed cardiac-specific markers cTnT and alpha-actin, and displayed transient outward K+ current (I-to), inwardly rectifying K+ current (I-K1) and delayed rectifier K+ current (I-KDR), which were typical of cardiomocytes. Other clones merely showed I-to current, and the current densities were different from those of cardiomyocytes. In contrast to the other clones, before induced with 5-azacytidine, the eight clones expressed early cardiac markers GATA4 and NKX2.5, but not cTnT, alpha-actin, CD44 and CD90, and had no potentials for adiopogenesis, osteogenesis or chondrogenesis after induction. Our data suggest that the subgroup of MSCs that contributes to cardiomyocytes differentiation is cardiac progenitor cells. Moreover, we show the preliminary purification of this population of cells with a high potential for cardiomyocytes differentiation using single-cell monoclonal technology. (C) 2011 Elsevier Inc. All rights reserved.